A comparison of hypoxia-inducible factor-α in surgically excised neovascular membranes of patients with diabetes compared with idiopathic epiretinal membranes in nondiabetic patients.
A comparison of hypoxia-inducible factor-α in surgically excised neovascular membranes of patients with diabetes compared with idiopathic epiretinal membranes in nondiabetic patients.
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DOI：
10.1097/iae.0b013e3181d6df09
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发表时间：
2010-10
期刊：
影响因子：
--
通讯作者：
Hinton DR
中科院分区：
文献类型：
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作者：
Lim JI;Spee C;Hinton DR
本研究的目的是首先确定糖尿病视网膜前膜中是否可检测到缺氧诱导因子-1 α（HIF-1 α），并比较HIF-1α在纤维血管增生性糖尿病视网膜病变膜和非糖尿病特发性视网膜前膜中的存在。12例增殖性糖尿病视网膜病变膜需要睫状体平坦部玻璃体切除术和9例非糖尿病患者的特发性视网膜前膜需要睫状体平坦部玻璃体切除术进行了这些膜的切除。在切除的膜上进行HIF-1α存在的免疫组织化学染色。确定每个标本的HIF-1α染色程度（1+、2+和3+量表）和染色的细胞位置。获得了所有患者的机构审查委员会批准和知情同意书。12例糖尿病视网膜前膜中有11例（92%）HIF-1α阳性，大多数细胞质染色强烈（2+至3+），偶见局灶性核阳性。5/9例（55%）非糖尿病视网膜前膜HIF-1α阳性，胞浆染色明显较弱（1+至2+），偶见局灶性点状核染色。与非糖尿病性特发性视网膜前膜相比，缺氧诱导因子-1 α在糖尿病性视网膜前膜中更常见且更强烈。
The purpose of this study was to first determine whether hypoxia-inducible factor-1α (HIF-1 α) was detectable in diabetic preretinal membranes and to compare the presence of HIF-1α in fibrovascular proliferative diabetic retinopathy membranes with nondiabetic, idiopathic, epiretinal membranes. Twelve patients with proliferative diabetic retinopathy membranes requiring pars plana vitrectomy and nine nondiabetic patients with idiopathic epiretinal membranes requiring pars plana vitrectomy underwent excision of these membranes. Immunohistochemical staining for the presence of HIF-1α was performed on the excised membranes. The degree of staining for HIF-1α (1+, 2+, and 3+ scale) and the cellular location of staining were determined for each specimen. Institutional Review Board approval and informed consent were obtained for all patients. Eleven of 12 (92%) diabetic preretinal membranes were positive for HIF-1α, and most had intense (2+ to 3+) cytoplasmic staining with occasional focal nuclear positivity. Five of 9 (55%) nondiabetic epiretinal membranes were positive for HIF-1α with significantly weaker cytoplasmic staining (1+ to 2+) with occasional focal punctuate nuclear staining. Hypoxia-inducible factor-1α is found more often and more intensely in diabetic preretinal membranes compared with nondiabetic idiopathic epiretinal membranes.