The desaturase1 gene affects reproduction before, during and after copulation in Drosophila melanogaster.
The desaturase1 gene affects reproduction before, during and after copulation in Drosophila melanogaster.
复制标题
去饱和酶 1 基因影响果蝇交配前、交配期间和交配后的繁殖。
DOI：
10.1080/01677063.2018.1559843
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Ferveur JF.
中科院分区：
文献类型：
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作者：
Nojima T;Chauvel I;Houot B;Bousquet F;Farine JP;Everaerts C;Yamamoto D;Ferveur JF.
在黑腹果蝇中，去饱和酶1 （desat1）是少数已知参与感觉通信的两个互补方面——信号发射和信号接收的基因之一。特别是，desat1对于雄性和雌性主要表皮信息素的生物合成都是必需的。它也与男性辨别性信息素的能力有关。这两个感觉交流方面的每一个都依赖于不同的假定的调节区域。在这里，我们使用了(i)由于插入/切除插入到desat1调控区域的转座基因组元件而产生的突变等位基因，以及（ii）用desat1调控区域制成的转基因用于靶向desat1 RNAi。这些遗传变异被用于研究几种与生殖相关的表型。特别地，我们比较了各种突变和转基因的desat1雌性在其后代发育命运方面的繁殖力。我们还比较了在各种desat1表达组织中desat1表达改变以及交配结束时无法脱离的成对果蝇的交配表现。此外，我们还研究了雄性果蝇性信息素识别改变的发育起源。我们试图绘制一些与这些生殖相关的表型相关的组织。鉴于desat1在许多脑神经细胞和生殖各方面所需的非神经组织中表达，我们的数据表明，该基因的调节已经进化到允许这种世界性物种的最佳繁殖和成功适应各种环境。
Desaturase1 (desat1) is one of the few genes known to be involved in the two complementary aspects of sensory communication — signal emission and signal reception — inDrosophila melanogaster. In particular, desat1 is necessary for the biosynthesis of major cuticular pheromones in both males and females. It is also involved in the male ability to discriminate sex pheromones. Each of these two sensory communication aspects depends on distinct desat1 putative regulatory regions. Here, we used (i) mutant alleles resulting from the insertion/excision of a transposable genomic element inserted in a desat1 regulatory region, and (ii) transgenics made with desat1 regulatory regions used to target desat1 RNAi. These genetic variants were used to study several reproduction-related phenotypes. In particular, we compared the fecundity of various mutant and transgenic desat1 females with regard to the developmental fate of their progeny. We also compared the mating performance in pairs of flies with altered desat1 expression in various desat1-expressing tissues together with their inability to disengage at the end of copulation. Moreover, we investigated the developmental origin of altered sex pheromone discrimination in male flies. We attempted to map some of the tissues involved in these reproduction-related phenotypes. Given that desat1 is expressed in many brain neurons and in non-neuronal tissues required for varied aspects of reproduction, our data suggest that the regulation of this gene has evolved to allow the optimal reproduction and a successful adaptation to varied environments in this cosmopolitan species.