The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis
The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis
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DOI：
10.1007/bf00564200
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发表时间：
1996-01-01
影响因子：
3.1
通讯作者：
Rafalski, A
中科院分区：
文献类型：
--
作者：
Powell, W;Morgante, M;Rafalski, A
通过评价RFLP（限制性片段长度多态性）、RAPD（随机扩增多态性DNA）、AFLP（扩增片段长度多态性）和SSR（简单重复序列，微卫星）标记在大豆种质资源分析中的应用，分析了它们的信息量（期望杂合度）、每个实验同时分析的位点数（多重比率）和评估种质资源间关系的有效性。SSR标记具有最高的期望杂合度（0.60），而AFLP标记具有最高的有效多重比率（19）。一个单一的参数，定义为标记指数，这是预期的杂合性和多重比率的产品，可以用来评估标记系统的整体效用。遗传相似性矩阵的比较表明，如果比较涉及栽培大豆（大豆）和野生大豆（大豆）加入，估计基于RFLP，AFLP和SSR高度相关，这些测定之间的一致性。然而，RAPD标记数据与其他标记系统获得的数据的相关性较低。这是因为RAPD产生了更高的种间相似性估计。如果比较涉及G. max，则标记系统之间的总体相关性显著较低。在G. max、RAPD和AFLP的相似性估计值之间的相关性比其他标记系统的相似性估计值之间的相关性更高。
The utility of RFLP (restriction fragment length polymorphism), RAPD (random-amplified polymorphic DNA), AFLP (amplified fragment length polymorphism) and SSR (simple sequence repeat, microsatellite) markers in soybean germplasm analysis was determined by evaluating information content (expected heterozygosity), number of loci simultaneously analyzed per experiment (multiplex ratio) and effectiveness in assessing relationships between accessions. SSR markers have the highest expected heterozygosity (0.60), while AFLP markers have the highest effective multiplex ratio (19). A single parameter, defined as the marker index, which is the product of expected heterozygosity and multiplex ratio, may be used to evaluate overall utility of a marker system. A comparison of genetic similarity matrices revealed that, if the comparison involved both cultivated (Glycine max) and wild soybean (Glycine soja) accessions, estimates based on RFLPs, AFLPs and SSRs are highly correlated, indicating congruence between these assays. However, correlations of RAPD marker data with those obtained using other marker systems were lower. This is because RAPDs produce higher estimates of interspecific similarities. If the comparisons involved G. max only, then overall correlations between marker systems are significantly lower. Within G. max, RAPD and AFLP similarity estimates are more closely correlated than those involving other marker systems.