Transcription factor Tbx6 plays a central role in fate determination between mesenchyme and muscle in embryos of the ascidian, Halocynthia roretzi.
Transcription factor Tbx6 plays a central role in fate determination between mesenchyme and muscle in embryos of the ascidian, Halocynthia roretzi.
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转录因子 Tbx6 在海鞘 (Halocynthia roretzi) 胚胎中间充质和肌肉之间的命运决定中发挥着核心作用。
DOI：
10.1111/dgd.12133
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
H.
中科院分区：
文献类型：
--
作者：
Kumano;G.;Negoro;N.;and Nishida;H.
发育命运取决于胚胎内周围细胞的其他命运，在发育过程中产生多样化的组织或细胞类型。在海鞘胚胎中，母源局部因子和诱导信号在胚胎发生过程中适当的空间和时间位置的命运决定中发挥着重要和合作的作用。在这里，为了阐明 Halocynthia roretzi 中成纤维细胞生长因子 (FGF) 信号诱导间充质过程中间充质和肌肉的命运决定机制，我们检查了转录因子 Tbx6 的功能，该因子作为母源局部肌肉分化决定因子 Macho-1 的关键介质。我们的结果表明，肌肉谱系细胞中 Tbx6 表达水平通过促进肌肉分化以及间充质命运抑制的正反馈调节而增加。此外，FGF信号使Tbx6转录活性和正反馈失活，从而诱导间充质谱系。综上所述，我们的发现表明 Tbx6 是决定间充质和肌肉命运的重要因素。
Developmental fates are determined in relation to other fates in surrounding cells within the embryo, creating diversified tissue or cell types in the course of development. In ascidian embryos, maternally localized factors and inductive signals play essential and cooperative roles in fate determination at appropriate spatial and temporal positions during embryogenesis. Here, to clarify fate determination mechanisms of mesenchyme and muscle during mesenchyme induction by the fibroblast growth factor (FGF) signal inHalocynthia roretzi, we examined the function of transcription factor Tbx6, which acts as a pivotal mediator of the maternally localized muscle differentiation determinant Macho‐1. Our results suggest that the level ofTbx6expression increases in muscle lineage cells through positive feedback regulation that promotes muscle differentiation as well as mesenchymal fate suppression. In addition, the FGF signal inactivated Tbx6 transcriptional activity and positive feedback, leading to induction of the mesenchymal lineage. Taken together, our finding suggests that Tbx6 is an important factor for determining mesenchyme and muscle fates.