DEVELOPMENTAL REGULATION OF HUMAN FETAL-TO-ADULT GLOBIN GENE SWITCHING IN TRANSGENIC MICE
DEVELOPMENTAL REGULATION OF HUMAN FETAL-TO-ADULT GLOBIN GENE SWITCHING IN TRANSGENIC MICE
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DOI：
10.1038/344309a0
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发表时间：
1990-03-22
期刊：
影响因子：
64.8
通讯作者：
STAMATOYANNOPOULOS, G
中科院分区：
文献类型：
--
作者：
ENVER, T;RAICH, N;STAMATOYANNOPOULOS, G
含有人胎儿(γ-)或成人(β-)珠蛋白基因的转基因小鼠在整个发育过程中都表达该基因，该基因与β-珠蛋白基因位点激活区(LAR)相连。相比之下，含有与胎儿和成人珠蛋白基因相关联的LAR的转基因小鼠表现出从胎儿到成人基因表达的正常发育转换。这表明人类从胎儿到成人的珠蛋白基因开关是通过LAR和γ或β珠蛋白基因之间的相互排斥作用来控制的，导致在任何给定时刻只有一个基因表达。
Transgenic mice containing a human fetal (γ-) or adult (β-) globin gene linked to the β-globin gene locus activation region (LAR) express the gene throughout development. By contrast transgenic mice containing LAR linked to both a fetal and an adult globin gene display the normal developmental switch from fetal to adult gene expression. This suggests that the human fetal-to-adult globin gene switch is controlled through a mutually exclusive interaction between LAR and either the γ- or β-globin gene, resulting in the expression of only one gene at any given moment.