1-DE MS and 2-D LC-MS analysis of the mouse bronchoalveolar lavage proteome
1-DE MS and 2-D LC-MS analysis of the mouse bronchoalveolar lavage proteome
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DOI：
10.1002/pmic.200500052
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发表时间：
2005-11-01
期刊：
影响因子：
3.4
通讯作者：
Garcia, JGN
中科院分区：
文献类型：
--
作者：
Guo, YR;Ma, SF;Garcia, JGN
支气管肺泡灌洗液（BALF）是一种复杂的蛋白质混合物，它代表了一种独特的临床有用的下呼吸道样本。许多蛋白质组学技术可用于表征复杂的生物混合物；然而，目前尚不清楚哪种技术可以提供更多关于已鉴定蛋白质数量和序列覆盖范围的信息。在本研究中，我们首先比较了两种常用的蛋白质组学方法，即二维LC microESI MS/MS和I-DE，然后是凝胶切片消化、肽提取和MS鉴定。其次，我们从小鼠BALF蛋白组中鉴定出297个独特的蛋白，无论物种，我们都将BALF蛋白组扩增了约三倍。
Bronchoalveolar lavage fluid (BALF) is a complex mixture of proteins, which represents a unique clinically useful sampling of the lower respiratory tract. Many proteomic technologies can be used to characterize complex biological mixtures; however, it is not yet clear which technology(s) provide more information regarding the number of proteins identified and sequence coverage. In this study, we initially compared two common proteomic approaches, 2-D LC microESI MS/MS and I-DE followed by gel slice digestion, peptide extraction and peptide identification by MS in characterization of the mouse BALF proteome; secondly, we identified 297 unique proteins from the mouse BALF proteome, greatly expanded the BALF proteome by about threefold regardless of species.