A standardized protocol for assessing regulators of pigmentation
A standardized protocol for assessing regulators of pigmentation
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DOI：
10.1006/abio.1999.4090
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发表时间：
1999-06-01
影响因子：
2.9
通讯作者：
Hearing, VJ
中科院分区：
文献类型：
--
作者：
Virador, VM;Kobayashi, N;Hearing, VJ
许多小组已经报道了化学或生物化合物对哺乳动物色素沉着的各种影响，但迄今为止，还没有标准化的方法建立必要和/或最佳参数来测试这些药物。开发了一种标准化的方法来筛选对色素沉着有潜在影响的化合物。该方案包括分析黑色素效应的基本参数，并允许进一步表征候选化合物，为其作用机制提供重要见解。在该方案中（称为STOPR，用于色素沉着调节因子的标准化测试），化合物最初使用纯化酪氨酸酶筛选，然后在培养的黑素细胞上进行测试，在用潜在生物活性化合物处理黑素细胞后，测量细胞增殖和活力，总黑色素积累和黑色素生成潜力。该协议是一个重要的第一步，在表征化学调节对黑素形成的影响。当确定了生物活性候选化合物后，可以在共培养和/或器官培养模型中进行药理学或其他商业应用测试，然后进行体内测试。作为该方法的应用，已知刺激和/或抑制黑色素生成的化合物（包括熊果苷、对苯二酚、曲酸、促黑素细胞激素和胸腺嘧啶二聚体）以及一些商业皮肤美白剂的结果被报道。
Varied effects of chemical or biological compounds on mammalian pigmentation have been reported by many groups, but to date, no standardized method has established necessary and/or optimal parameters for testing such agents. A standardized method has been developed to screen compounds with potential effects on pigmentation. The protocol comprises basic parameters to analyze melanogenic effects and allows for further characterization of candidate compounds, providing important insights into their mechanism of action. In this protocol (termed STOPR, for standardized testing of pigmentation regulators), compounds are initially screened using purified tyrosinase and are then tested on melanocytes in culture, After treatment of melanocytes with potentially bioactive compounds, cell proliferation and viability, total melanin accumulated, and melanogenic potential are measured. This protocol is an important first step in characterizing chemical regulation of effects on melanogenesis. When bioactive candidate compounds are identified, testing may proceed for pharmacological or otherwise commercial applications in coculture and/or organ culture models followed by in vivo testing. As an application of this method, results for compounds known to stimulate and/or inhibit melanogenesis (including arbutin, hydroquinone, kojic acid, melanocyte-stimulating hormone, and thymidine dimers) as well as some commercial skin whiteners are reported.