Genomic organization and expression of the rearranged REL proto-onicogene in the human B-cell lymphoma cell line RC-K8
Genomic organization and expression of the rearranged REL proto-onicogene in the human B-cell lymphoma cell line RC-K8
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DOI：
10.1002/gcc.10051
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发表时间：
2002-05-01
影响因子：
3.7
通讯作者：
Gilmore, TD
中科院分区：
文献类型：
--
作者：
Kalaitzidis, D;Gilmore, TD
人大B细胞淋巴瘤细胞系RC-K8具有重排的REL基因座，其被转录成嵌合mRNA，称为NRG-NRG（非Rel基因）。通过分析最近完成的人类基因组序列，我们发现正常的REL和NRG基因座在2号染色体上相隔约28兆碱基对，这表明缺失在RC-K8细胞中产生了NRG基因座。使用基于计算机和分子的方法，我们已经确定了RC-K8细胞中改变的REL基因座的结构。NRG转录本由7个REL外显子和6个NRG衍生的外显子编码。直接DNA测序已经鉴定了RC-K8细胞中的NRG-NRG融合位点。我们还表明，野生型c-Rel和c-Rel-Nrg蛋白的表达，并在一个复杂的RC-K8细胞。此外，与c-Rel一样，c-Rel-Nrg在培养的成纤维细胞中过表达时是一种细胞质蛋白，并且可以在体外与kappaB DNA位点结合。(C)2002 Wiley-Liss，Inc.
The human large B-cell lymphoma cell line RC-K8 has a rearranged REL locus that is transcribed into a chimeric mRNA, termed REL-NRG (Non-Rel Gene). By analyzing the recently completed human genome sequence, we have found that the normal REL and NRG loci are separated by approximately 28 megabase pairs on chromosome 2, suggesting that a deletion created the REL-NRG locus in RC-K8 cells. Using computer-based and molecular approaches, we have determined the structure of the altered REL locus in RC-K8 cells. The REL-NRG transcript is encoded by 7 REL exons and 6 NRG-derived exons. Direct DNA sequencing has identified the site of the REL-NRG fusion in RC-K8 cells. We also show that both wild-type c-Rel and c-Rel-Nrg proteins are expressed and in a complex in RC-K8 cells. Furthermore, like c-Rel, c-Rel-Nrg is a cytoplasmic protein when overexpressed in fibroblasts in culture and can bind to a kappaB DNA site in vitro. (C) 2002 Wiley-Liss, Inc.