Expression of CD80 and CD86 on B cells during coxsackievirus B3-induced acute myocarditis
Expression of CD80 and CD86 on B cells during coxsackievirus B3-induced acute myocarditis
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柯萨奇病毒B3诱导的急性心肌炎B细胞CD80和CD86的表达
DOI：
10.5114/ceji.2019.92786
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发表时间：
2020-01
期刊：
影响因子：
--
通讯作者：
weifeng Wu
中科院分区：
文献类型：
--
作者：
yanlan Huang;bin Wei;xingcui Gao;yan Deng;weifeng Wu
病毒性心肌炎（VMC）的发病机制尚不清楚，但许多研究表明，VMC与过度免疫应答有关。CD80和CD86是重要的共刺激分子，在自身免疫中起关键作用。然而，CD80+/CD86+ B细胞是否参与急性VMC的发病机制尚不清楚。材料与方法采用柯萨奇病毒B3 （CVB3）腹腔注射感染雄性C57BL/6小鼠，建立VMC模型。对照组小鼠腹腔注射磷酸盐缓冲盐水。注射后1周和2周，用血红素和伊红染色评估心脏组织的组织病理学变化。流式细胞术检测脾脏CD80+/CD86+ B细胞的频率。结果VMC中CD80+ B细胞频率明显升高，CD86+ B细胞频率明显降低。此外，CD80+ B细胞的频率与VMC的严重程度有关。结论CD80+/CD86+B细胞参与了VMC的发病过程，且CD80+B细胞比CD86+B细胞更重要。
Introduction The pathogenesis of viral myocarditis (VMC) is unclear, but many studies have shown that VMC is associated with an excessive immune response. CD80 and CD86 are important costimulatory molecules that play a critical role in autoimmunity. However, whether CD80+/CD86+ B cells participate in the pathogenesis of acute VMC is unknown. Material and methods Male C57BL/6 mice were infected by intraperitoneal injection with coxsackievirus B3 (CVB3) to establish a VMC model. Control mice were administered phosphate-buffered saline intraperitoneally. At one week and two weeks post injection, histopathological changes in heart tissue were assessed with haematoxylin and eosin staining. The frequency of splenic CD80+/CD86+ B cells was measured with flow cytometry. Results The frequency of CD80+ B cells was significantly increased in VMC, while the frequency of CD86+ B cells was significantly decreased. Furthermore, the frequency of CD80+ B cells related to the severity of VMC. Conclusions These data show that CD80+/CD86+B cells are involved in the pathogenesis of VMC, with CD80+B cells being more important than CD86+B cells.
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DOI：
--
发表时间：
1996-10
期刊：
The Canadian journal of cardiology
影响因子：
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作者：
Peter Liu;Tami A. Martino;M. Opavsky;Josef M. Penninger
通讯作者：
Peter Liu;Tami A. Martino;M. Opavsky;Josef M. Penninger
影响因子：
9.3
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DOI：
10.1146/annurev.cb.02.110186.001311
发表时间：
1986
期刊：
Annual review of cell biology
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DOI：
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发表时间：
2000-05
期刊：
The Journal of Immunology
影响因子：
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作者：
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通讯作者：
N. Pardigon;C. Cambouris;N. Bercovici;F. Lemaître;R. Liblau;P. Kourilsky
影响因子：
64.5
作者：
KUCHROO, VK;DAS, MP;GLIMCHER, LH
通讯作者：
GLIMCHER, LH