Preferential incorporation of coloured-carotenoids occurs in the LH2 complexes from non-sulphur purple bacteria under carotenoid-limiting conditions
Preferential incorporation of coloured-carotenoids occurs in the LH2 complexes from non-sulphur purple bacteria under carotenoid-limiting conditions
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DOI：
10.1007/s11120-005-3481-0
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发表时间：
2005-11-01
影响因子：
3.7
通讯作者：
Cogdell, RJ
中科院分区：
文献类型：
--
作者：
Gall, A;Henry, S;Cogdell, RJ
培养嗜酸红假单胞菌（Rps）和Rps的效果。研究了不同浓度的类胡萝卜素（Car）生物合成抑制剂二苯胺（DPA）对palustris生长的影响。DPA浓度低于最大的生长诱导Car限制。对DPA的确切反应取决于物种。然而，两个Rps。嗜酸菌和Rps。palustris的反应是优先将有限数量的彩色汽车纳入其LH2复合物，而牺牲RC-LH1复合物。随着DPA的抑制作用变得更加严重，共轭C=C键数量减少的car的百分比增加。利用吸收光谱、荧光光谱、CD光谱和拉曼光谱研究了这种改变的Car成分对天线配合物结构和功能的影响。结果表明，尽管Car分子的存在对LH2配合物的稳定性很重要，但许多不同Car的存在可以维持整体的天然结构。
The effect of growing Rhodopseudomonas (Rps.) acidophila and Rps. palustris in the presence of different concentrations of the carotenoid (Car) biosynthetic inhibitor diphenylamine (DPA) has been investigated. Growth with sub-maximal concentrations of DPA induces Car limitation. The exact response to DPA is species dependent. However, both Rps. acidophila and Rps. palustris respond by preferentially incorporating the limiting amount of coloured Cars into their LH2 complexes at the expense of the RC-LH1 complexes. As inhibition by DPA becomes more severe there is an increase in the percentage of Cars with reduced numbers of conjugated C=C bonds. The effect of this changed Car composition on the structure and function of the antenna complexes has been investigated using absorption, fluorescence, CD and Raman spectroscopies. The results show that although the presence of Car molecules is important for the stability of the LH2 complexes that the overall native structure can be maintained by the presence of many different Cars.