RAPID ONSET AND CALCIUM INDEPENDENCE OF THE GAP JUNCTION UNCOUPLING INDUCED BY HEPTANOL IN CULTURED HEART-CELLS
RAPID ONSET AND CALCIUM INDEPENDENCE OF THE GAP JUNCTION UNCOUPLING INDUCED BY HEPTANOL IN CULTURED HEART-CELLS
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DOI：
10.1007/bf00374154
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发表时间：
1995-01-01
影响因子：
4.5
通讯作者：
DELEZE, J
中科院分区：
文献类型：
--
作者：
BASTIDE, B;HERVE, JC;DELEZE, J
在从新生大鼠心室分离并培养2-3天的成对肌细胞中，研究了脂肪醇庚醇可逆性中断间隙连接通讯的动力学和可能的胞质Ca 2+浓度增加的介导。通过测量细胞间电导和双全细胞电压钳方法，或通过光漂白后荧光恢复（gap FRAP）技术测量染料扩散速率常数来估计连接通讯。观察到电偶联被庚醇（1-3 mM）突然中断（小于0.5s）。胞浆Ca 2+浓度不受影响，即使在饱和庚醇浓度。庚醇去除允许间隙连接通道的逐渐重新打开，如细胞间电导的恢复曲线所示，其在90秒内完成90%。这些数据与庚醇与通道蛋白或其脂质环境的直接相互作用一致。
The kinetics of the reversible interruption of gap junction communication by the aliphatic alcohol heptanol and the possible mediation of an increase of the cytosolic Ca2+ concentration have been investigated in pairs of myocytes dissociated from neonatal rat ventricles and cultured for 2-3 days. Junctional communication was estimated by measuring either the cell-to-cell electrical conductance with a double whole-cell voltage-clamp method, or the rate constant of dye diffusion with the fluorescence recovery after photo-bleaching (gap FRAP) technique. Electrical coupling was seen to be abruptly interrupted (in less than 0.5 s) by heptanol (1-3 mM). The cytosolic Ca2+ concentration was not affected, even at a saturating heptanol concentration. Heptanol removal allowed a gradual re-opening of gap junctional channels, as shown by the recovery curve of the cell-to-cell conductance, which is 90% complete within 90 s. These data are consistent with a direct interaction of heptanol with channel proteins or with their lipid environment.