The cys-loop ligand-gated ion channel gene superfamily of the red flour beetle, Tribolium castaneum.
The cys-loop ligand-gated ion channel gene superfamily of the red flour beetle, Tribolium castaneum.
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红粉甲虫的cys-loop配体门控离子通道基因tribolium castaneum。
DOI：
10.1186/1471-2164-8-327
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发表时间：
2007-09-19
期刊：
影响因子：
4.4
通讯作者：
Sattelle, David B
中科院分区：
文献类型：
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作者：
Jones, Andrew K;Sattelle, David B
cys-loop配体门控离子通道（cys-loop LGIC）超家族成员介导化学神经传递，作为治疗阿尔茨海默病等神经系统疾病药物的潜在靶点被广泛研究。昆虫cyys -loop lgic也引起了人们的兴趣，因为它们是非常成功的杀虫剂的目标。赤粉甲虫（Tribolium castaneum）是储粮农产品的主要害虫，也是研究发展的重要模式生物。作为castaneum基因组测序工作的一部分，我们确定了甲虫cys-loop llic超家族，这是继果蝇和蜜蜂之后被描述的第三个昆虫超家族，也是由24个基因组成的最大的昆虫超家族。与果蝇和Apis一样，Tribolium具有由乙酰胆碱、γ-氨基丁酸（GABA）、谷氨酸和组胺以及果蝇ph敏感氯离子通道亚基（pHCl） CG8916和CG12344的同源物控制的离子通道。与果蝇和Apis类似，尽管甲虫的RDL和葡萄糖同源物拥有更多的外显子3变体，但Tribolium的cys-loop LGIC多样性通过选择性剪接而扩大。此外，在两个Tribolium烟碱乙酰胆碱受体亚基Tcasα6和Tcasβ1中也观察到RNA A-to-I编辑。在D. melanogaster、a . mellifera和Heliothis virescens中，Tcasα6的编辑在进化上是保守的，而Tcasβ1的编辑位点迄今仅在甲虫中观察到。我们的研究结果表明，在不同的昆虫物种中，cys-loop LGIC超家族保持紧密，基因数量仅发生微小变化。然而，选择性剪接、RNA编辑和不同亚基的存在拓宽了cys环LGIC蛋白质组，并产生了物种特异性受体同种异构体。这些发现增强了我们对cys-loop LGIC功能基因组学的认识，并为开发针对重要农业害虫的改良杀虫剂提供了有益的基础。
Members of the cys-loop ligand-gated ion channel (cys-loop LGIC) superfamily mediate chemical neurotransmission and are studied extensively as potential targets of drugs used to treat neurological disorders such as Alzheimer's disease. Insect cys-loop LGICs are also of interest as they are targets of highly successful insecticides. The red flour beetle, Tribolium castaneum, is a major pest of stored agricultural products and is also an important model organism for studying development. As part of the T. castaneum genome sequencing effort, we have characterized the beetle cys-loop LGIC superfamily which is the third insect superfamily to be described after those of Drosophila melanogaster and Apis mellifera, and also the largest consisting of 24 genes. As with Drosophila and Apis, Tribolium possesses ion channels gated by acetylcholine, γ-amino butyric acid (GABA), glutamate and histamine as well as orthologs of the Drosophila pH-sensitive chloride channel subunit (pHCl), CG8916 and CG12344. Similar to Drosophila and Apis, Tribolium cys-loop LGIC diversity is broadened by alternative splicing although the beetle orthologs of RDL and GluCl possess more variants of exon 3. Also, RNA A-to-I editing was observed in two Tribolium nicotinic acetylcholine receptor subunits, Tcasα6 and Tcasβ1. Editing in Tcasα6 is evolutionarily conserved with D. melanogaster, A. mellifera and Heliothis virescens, whereas Tcasβ1 is edited at a site so far only observed in the beetle. Our findings reveal that in diverse insect species the cys-loop LGIC superfamily has remained compact with only minor changes in gene numbers. However, alternative splicing, RNA editing and the presence of divergent subunits broadens the cys-loop LGIC proteome and generates species-specific receptor isoforms. These findings on Tribolium castaneum enhance our understanding of cys-loop LGIC functional genomics and provide a useful basis for the development of improved insecticides that target an important agricultural pest.