Enzyme stabilization via cross-linked enzyme aggregates.
Enzyme stabilization via cross-linked enzyme aggregates.
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DOI：
10.1007/978-1-60761-895-9_11
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发表时间：
2011
影响因子：
--
通讯作者：
M. N. Gupta;Smita Raghava
中科院分区：
文献类型：
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作者：
M. N. Gupta;Smita Raghava
蛋白质沉淀物通过交联剂（最常用的是戊二醛）的广泛交联，即不溶于乙醇的酶制剂，称为交联酶聚集体（CLEA）。CLEA在常规水性介质以及非水性介质中均显示出高稳定性和性能。它们在相当高的温度下也是稳定的。可以制备具有多于一种酶活性的CLEA，并且这种CLEA被称为组合CLEA或多用途CLEA。交联程度通常影响它们的形态、稳定性、活性和对映选择性。
Extensive cross-linking of a precipitate of a protein by a cross-linking reagent (glutaraldehyde has been most commonly used) createsaninsoluble enzyme preparation called cross-linked enzyme aggregates (CLEAs). CLEAs show high stability and performance in both conventional aqueous media as well as nonaqueous media. These are also stable at fairly high temperatures. CLEAs having more than one kind of enzyme activity can be prepared and such CLEAs are called combi-CLEAs or multipurpose CLEAs. Extent of cross-linking often influences their morphology, stability, activity, and enantioselectivity.