Metagenomics reveals divergent functional profiles of soil carbon and nitrogen cycling under long-term addition of chemical and organic fertilizers in the black soil region
Metagenomics reveals divergent functional profiles of soil carbon and nitrogen cycling under long-term addition of chemical and organic fertilizers in the black soil region
复制标题
宏基因组学揭示了黑土地区长期添加化肥和有机肥下土壤碳氮循环的不同功能特征
DOI：
10.1016/j.geoderma.2022.115846
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发表时间：
2022-03-28
期刊：
影响因子：
6.1
通讯作者：
Wang, Guanghua
中科院分区：
文献类型：
--
作者：
Hu, Xiaojing;Gu, Haidong;Wang, Guanghua
不同施肥制度对涉及养分循环的土壤微生物功能潜力的长期影响在很大程度上仍然未知。本研究采用宏基因组测序技术，研究了东北黑土区南、中、北三样地长期施用化学和有机施肥对土壤微生物C、N循环的影响。结果表明，生物地理距离对土壤碳氮循环的微生物功能特征影响最大，且施用有机肥对土壤碳氮循环的影响显著。有机肥增加了携带C和N循环基因的变形菌和plantomcetes的相对丰度，同时抑制了Verrucomicrobia等贫营养菌群的生长。化学施肥增加了与甲烷氧化有关的基因丰度，但对土壤碳的降解和固定影响不大。相反，粪肥特别是有机肥配施显著降低了参与碳固定的coc（还原性乙酰- coa途径）和coxS （CO氧化）的丰度，而提高了参与碳固定的icd （rTCA循环）的丰度。此外，化学施肥增加了参与土壤氮降解、硝化和厌氧氨氧化的基因丰度，而粪肥则有利于整个黑土吸收和异化硝酸盐还原的功能潜力。但CFM显著促进了土壤反硝化潜力，这可能是由于过量的N输入，这可能导致该地区土壤N通过排放氮气而流失。此外，施肥导致的土壤磷含量的大幅增加主要影响了碳和氮循环剖面、功能基因丰度和微生物类群。此外，C和N循环基因之间的不同相关性表明黑土中C和N代谢潜力存在协同或拮抗的相互作用。总的来说，本研究深入了解了长期化学和有机施肥下不同的微生物功能潜力，这可能对黑土区农业生态系统的土壤养分循环产生可预测的影响。
The long-term effects of different fertilization regimes on the microbial functional potential of soils involving nutrient cycling remain largely unknown. Here, metagenomic sequencing was applied to investigate the influences of long-term chemical and organic fertilization on soil microbial C and N cycling across southern, middle and northern sites of black soil region in Northeast China. The results showed that biogeographic distance induced the most influential on the microbial functional profiles of soil C and N cycling, and significant effects of manure fertilization were detected across three experimental sites. Organic fertilization enriched the relative abundances of Proteobacteria and Planctomycetes that carry C and N cycling genes, while inhibited the growth of oligotrophic groups such as Verrucomicrobia. Chemical fertilization increased the gene abundances involved in methane oxidation, but had little effect on soil C degradation and fixation. Contrarily, manure fertilization, particularly the combination of chemical and organic fertilizers (CFM), significantly decreased the abundance of cooC (reductive acetyl-CoA pathway) and coxS (CO oxidation) while enhanced the abundance of icd (rTCA cycle), which are involved in C fixation. Additionally, chemical fertilization enriched the gene abundance that involved in soil N degradation, nitrification and anammox, whereas manure fertilization was beneficial for the functional potentials of assimilatory and dissimilatory nitrate reductions across the black soils. However, CFM significantly promoted the soil denitrification potential, possibly due to excess N input, which might result in soil N loss via the emission of nitrogenous gas in this region. Furthermore, the substantial enhancement in soil P contents induced by manure addition predominantly affected the C and N cycling profiles, abundance of functional genes and microbial taxa. Moreover, diverse correlations between C and N cycling genes suggested the synergetic or antagonistic interactions of C and N metabolic potentials in the black soils. Overall, this study provided in-depth insights into distinct microbial functional potentials under long-term chemical and organic fertilization that may have predictable consequences for soil nutrient cycling in agroecosystems of black soil region.