920 MHz ultra-high field NMR approaches to structural glycobiology
920 MHz ultra-high field NMR approaches to structural glycobiology
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DOI：
10.1016/j.bbagen.2007.11.014
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发表时间：
2008-03-01
影响因子：
3
通讯作者：
Yamaguchi, Yoshiki
中科院分区：
文献类型：
--
作者：
Kato, Koichi;Sasakawa, Hiroaki;Yamaguchi, Yoshiki
虽然核磁共振波谱法有很大的潜力，为我们提供详细的结构信息，寡糖和糖缀合物，碳水化合物的核磁共振分析已受到严重的光谱重叠和不足的构象限制。最近，超高场NMR光谱仪已成为可用于生物大分子的结构分析的应用。在这里，我们表明，超高场不仅提供了增加的灵敏度和化学位移分散，但也提供了独特的信息化学交换和松弛的潜在好处，通过显示NMR光谱数据的寡糖，糖蛋白和糖脂系统记录在21.6 T的磁场（对应于920 MHz H-1观测频率）。超高场核磁共振波谱技术结合糖库和稳定同位素标记技术将为糖结构生物学的研究开辟新的领域。(C)2007 Elsevier B. V.保留所有权利。
Although NMR spectroscopy has great potential to provide us with detailed structural information on oligosaccharides and glycoconjugates, the carbohydrate NMR analyses have been hampered by the severe spectral overlapping and the insufficiency of the conformational restraints. Recently, ultra-high field NMR spectrometers have become available for applications to structural analyses of biological macromolecules. Here we demonstrate that ultra-high fields offer not only increases in sensitivity and chemical shift dispersion but also potential benefits for providing unique information on chemical exchange and relaxation, by displaying NMR spectral data of oligosacchadde, glycoprotein, and glycolipid systems recorded at a 21.6 T magnetic field (corresponding to 920 MHz H-1 observation frequency). The ultra-high field NMR spectroscopy combined with sugar library and stable-isotope labeling approaches will open new horizons in structural glycobiology.(C) 2007 Elsevier B.V. All rights reserved.