Pluripotent neural crest stem cells in the adult hair follicle
Pluripotent neural crest stem cells in the adult hair follicle
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DOI：
10.1002/dvdy.20129
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发表时间：
2004-10-01
影响因子：
2.5
通讯作者：
Szeder, V
中科院分区：
文献类型：
--
作者：
Sieber-Blum, M;Grim, M;Szeder, V
我们报道多能神经嵴干细胞的存在。成年哺乳动物的毛囊大量的神经嵴细胞存在于从突起到毛囊基部基质的外根鞘中。从成年鼠须卵泡中提取的膨大外植体可产生迁移的神经嵴细胞，这些细胞在克隆培养中形成由一千多个细胞组成的菌落。克隆细胞包含神经元、平滑肌细胞、罕见的雪旺细胞和黑素细胞，显示出克隆形成细胞的多能性。神经调节蛋白-1和骨形态发生蛋白-2分别实现了向雪旺细胞和软骨细胞的定向分化。体外连续克隆显示出自我更新能力。总之，这些数据表明，成年小鼠须毛囊含有多能神经嵴干细胞，称为表皮神经嵴细胞（eNCSC）。由于其高度的固有可塑性和易于在皮肤中获取，eNCSC是多种细胞治疗范式的有希望的候选者。(C) 2004 Wiley-Liss, Inc。
We report the presence of pluripotent neural crest stem cells in. the adult mammalian hair follicle. Numerous neural crest cells reside in the outer root sheath from the bulge to the matrix at the base of the follicle. Bulge explants from adult mouse whisker follicles yield migratory neural crest cells, which in clonal culture form colonies consisting of over a thousand cells. Clones contain neurons, smooth muscle cells, rare Schwann cells and melanocytes, demonstrating pluripotency of the clone-forming cell. Targeted differentiation into Schwann cells and chondrocytes was achieved with neuregulin-1 and bone morphogenetic protein-2, respectively. Serial cloning in vitro demonstrated self-renewal capability. Together, the data show that the adult mouse whisker follicle contains pluripotent neural crest stem cells, termed epidermal neural crest cells (eNCSC). eNCSC are promising candidates for diverse cell therapy paradigms because of their high degree of inherent plasticity and due to their easy accessibility in the skin. (C) 2004 Wiley-Liss, Inc.