Hypertriglyceridemia in female rats during pregnancy induces obesity in male offspring via altering hypothalamic leptin signaling.
Hypertriglyceridemia in female rats during pregnancy induces obesity in male offspring via altering hypothalamic leptin signaling.
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怀孕期间雌性大鼠的高甘油三酯血症通过改变下丘脑瘦素信号传导诱导雄性后代肥胖
DOI：
10.18632/oncotarget.18519
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发表时间：
2017-08-08
期刊：
影响因子：
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通讯作者：
Huang HF
中科院分区：
文献类型：
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作者：
Rahman TU;Ullah K;Ke ZH;Guo MX;Jin LY;Ren J;Zhou YZ;Cheng Y;Dong XY;Pang HY;Wang TT;Sheng JZ;Huang HF
母亲肥胖影响儿童的长期发育和健康。然而，这一过程中涉及的机制仍不确定。在目前的研究中，我们探索了孕期过量喂养高脂饮食是否改变了F1代雌性大鼠的代谢表型，并验证了下丘脑瘦素信号的作用。分析瘦素反应性和磷酸化信号转导与激活转录3(PSTAT3)免疫阳性神经元的数量。本文观察了神经肽Y在下丘脑弓状核和孤束核中的分布。高脂饮食母亲的甘油三酯和瘦素水平升高。与Chow-F1相比，高脂饲料F1子代(HDF-F1)神经肽Y阳性细胞体和神经元的数量显著增加。瘦素能显著降低CHOW-F1弓状核神经元的摄食量，增加pSTAT3的表达水平。然而，瘦素对食物摄入量没有任何影响，并降低了HDF-F1弓状核神经元中PSTAT3的表达水平。从目前的多米诺骨牌效应，我们得出结论，母亲在怀孕期间暴露于高脂肪饮食可能通过改变下丘脑瘦素信号将肥胖表型传递给后代。
Maternal obesity influence the child's long-term development and health. Though, the mechanism concerned in this process is still uncertain. In the present study, we explored whether overfeeding of a high-fat diet during pregnancy in female rats altered metabolic phenotypes in an F1 generation and authenticated the contribution of hypothalamic leptin signaling. Leptin responsiveness and the number of immunopositive neurons for phosphorylated signal transducer and activator transcription 3 (pSTAT3) were analyzed. Neuropeptide Y in the arcuate nucleus of the hypothalamus and in nucleus tractus solitaries was examined. Triglycerides and leptin levels were increased in the high-fat diet mother. The number of neuropeptide Y positive cell bodies and neurons was significantly increased in the high-fat diet-F1 offspring (HDF-F1) as compared to Chow-F1. Leptin administration significantly decreased the food intake and increased the pSTAT3 expression levels in neurons in the arcuate nucleus of Chow-F1. However, leptin did not show any effect on food intake and had a reduced effect on pSTAT3 expression levels in neurons in the arcuate nucleus of HDF-F1. From the present domino effect, we conclude that mothers exposed to high-fat diet during pregnancy may pass the obese phenotype to the succeeding generation via altering hypothalamic leptin signaling.