Female influence on pre- and post-copulatory sexual selection and its genetic basis in Drosophila melanogaster.
Female influence on pre- and post-copulatory sexual selection and its genetic basis in Drosophila melanogaster.
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DOI：
10.1111/j.1365-294x.2011.05253.x
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发表时间：
2011-10
影响因子：
4.9
通讯作者：
Fiumera AC
中科院分区：
文献类型：
--
作者：
Giardina TJ;Beavis A;Clark AG;Fiumera AC
雌性果蝇的遗传变异可能会影响交配前和交配后的性选择结果。在这里，我们使用关联测试来调查10个候选女性基因的自然变异对女性生殖的影响。对91个2号染色体替换系中的雌性进行了评分，以了解影响交配前后性别选择的表型，如交配和交配率，使用第二个雄性的精子交配的倾向，以及生育能力的衡量标准。所有测定的性状的表型变异都有显著的遗传贡献。对91个品系中的10个候选基因进行重新测序，产生了68个非同义多态，并测试了这些多态与所测表型的相关性。我们发现了12个显著的关联(P<；0.01)。推测的丝氨酸蛋白酶同源基因CG9897和推测的气味结合蛋白CG11797的多态与女性交配的倾向相关，并满足P<；0.05的实验意义。其他几个关联，包括那些同时影响生育力和女性发育率的关联表明，精子存储可能是减轻女性对性选择的影响的一个重要因素。
Genetic variation among females is likely to influence the outcome of both pre- and post-copulatory sexual selection in Drosophila melanogaster. Here we use association testing to survey natural variation in 10 candidate female genes for their effects on female reproduction. Females from 91 chromosome 2 substitution lines were scored for phenotypes affecting pre- and post-copulatory sexual selection such as mating and remating rate, propensity to use sperm from the second male to mate, and measures of fertility. There were significant genetic contributions to phenotypic variation for all the traits measured. Resequencing of the 10 candidate genes in the 91 lines yielded 68 nonsynonymous polymorphisms which were tested for associations with the measured phenotypes. Twelve significant associations (markerwise P < 0.01) were identified. Polymorphisms in the putative serine protease homolog CG9897 and the putative odorant binding protein CG11797 associated with female propensity to remate and met an experimentwise significance of P < 0.05. Several other associations, including those impacting both fertility and female remating rate suggest that sperm storage might be an important factor mitigating female influence on sexual selection.