Electrospray ionization source with a rear extractor
Electrospray ionization source with a rear extractor
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DOI：
10.1002/jms.4072
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发表时间：
2018-05-01
影响因子：
2.3
通讯作者：
Hiraoka, Kenzo
中科院分区：
文献类型：
--
作者：
Chen, Lee Chuin;Tsutsui, Satoru;Hiraoka, Kenzo
一种新的电喷雾源的设计是通过具有一个提取器电极放置在发射器尖端后面1至2毫米。提取器作为单个装置集成到喷雾器主体中。使用绝缘管将发射器与提取器隔离并输送鞘气用于电喷雾。发射极处的电场强度主要由针和提取器之间的相对位置和电势决定;因此，喷雾条件不受喷雾器相对于离子采样入口的位置或方向的变化的影响。这种设计允许使用低得多的工作电压，并通过保持电场参数恒定来促进喷雾器位置的优化。使用内径为150和310 μ m的发射极毛细管，即使将发射极与离子入口的距离延长到> 70 mm，在发射极电位为2-kV时仍能获得较强的离子信号。还证明了使用2个相反发射极极性的基于提取器的电喷雾的蛋白质离子的电荷减少。
A new electrospray source design is introduced by having an extractor electrode placed at 1 to 2mm behind the emitter tip. The extractor was integrated into the sprayer body as a single device. An insulating tube was used to isolate the emitter from the extractor and to deliver the sheath gas for the electrospray. The electric field strength at the emitter was primarily determined by the relative position and the potential between the needle and the extractor; therefore, the spraying condition was insusceptible to the change of sprayer position or orientation with respect to the ion sampling inlet. Such design allowed the use of much lower operating voltage and facilitated the optimization of sprayer position by keeping the electric field parameter constant. Using an emitter capillary of 150 and 310m in inner and outer diameters, strong ion signal could still be acquired with 2-kV emitter potential even if the distance between the emitter and ion inlet was extended to >70mm. Charge reduction of protein ions using 2 extractor-based electrosprays of opposite emitter polarities was also demonstrated.