PRIMARY CULTURES OF NORMAL AND TUMORAL HUMAN OVARIAN EPITHELIUM - A POWERFUL TOOL FOR BASIC MOLECULAR STUDIES
PRIMARY CULTURES OF NORMAL AND TUMORAL HUMAN OVARIAN EPITHELIUM - A POWERFUL TOOL FOR BASIC MOLECULAR STUDIES
复制标题
DOI：
10.1006/excr.1994.1346
复制
发表时间：
1994-12-01
影响因子：
3.7
通讯作者：
MESMASSON, AM
中科院分区：
文献类型：
--
作者：
LOUNIS, H;PROVENCHER, D;MESMASSON, AM
为了开始描述卵巢癌发生中重要的细胞和分子事件，我们开发了一种简单而快速的方法来建立源自卵巢良性肿瘤、恶性肿瘤和腹水的原代培养物，这些培养物代表了它们所源自的原始临床材料。从收集的 23 份卵巢上皮性腹水中，13 份成功培养，细胞平均存活 7 至 8 代。从65个实体上皮性卵巢肿瘤（良性和恶性）中，将36个进行培养，对于来自良性肿瘤的培养物平均传代6次，对于恶性肿瘤的培养物平均传代11或12次。使用抗细胞角蛋白抗体通过形态学和组织化学分析将细胞本质上评分为上皮细胞。培养物，尤其是来自实体瘤的培养物，有时会出现类似成纤维细胞的污染，但很快就会得到解决。我们进行了有限的分子分析，以表征我们已建立的群体的起源，并证明这些培养物在分子研究中的有用性。 (C) 1994 学术出版社
To begin delineating the cellular and molecular events that are important in ovarian carcinogenesis, we have developed a simple and rapid method for the establishment of primary cultures derived from benign tumors, malignant tumors, and ascites of the ovary that are representative of the original clinical material from which they are derived. From 23 ovarian epithelial ascites collected, 13 were successfully established in culture and cells survived an average of 7 to 8 passages. From 65 solid epithelial ovarian tumors (benign and malignant) 36 were cultured for an average of 6 passages for cultures derived from benign tumors and 11 or 12 passages in the case of malignant tumors. Cells were scored as epithelial in nature by morphology and histochemical analysis using anti-cytokeratin antibodies. Cultures, especially those derived from solid tumors, sometimes displayed fibroblastic-like contamination which was quickly resolved. We include limited molecular analyses both to characterize the origin of the populations we have established as well as to demonstrate the usefulness of these cultures in molecular studies. (C) 1994 Academic Press,Inc.