Dynamic contrast-enhanced computed tomography lymphangiography with intranodal injection of water-soluble iodine contrast media in microminipig: imaging protocol and feasibility
Dynamic contrast-enhanced computed tomography lymphangiography with intranodal injection of water-soluble iodine contrast media in microminipig: imaging protocol and feasibility
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DOI：
10.1007/s00330-020-07031-0
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发表时间：
2020-06-26
影响因子：
5.9
通讯作者：
Matsuo, Masayuki
中科院分区：
文献类型：
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作者：
Tanahashi, Yukichi;Iwasaki, Ryota;Matsuo, Masayuki
目的探讨微型猪淋巴结内动态增强ct淋巴管造影（DCCTL）的最佳成像方案及可行性。方法动物研究与福利委员会批准。5只雌性微型猪在腹股沟淋巴结以0.3 mL/min的速度注射碘造影剂0.1 mL/kg后进行DCCTL，三种不同浓度的碘：1,75 mg /mL组；2组150 mgI/mL；3组300 mgI/mL。测量静脉角、胸导管、乳糜池、髂淋巴管、髂淋巴结的CT值；对比前后CT值的增加作为对比增强指数（CEI）进行评估。对显示最高CEI的多检测器行CT （MDCT）和体积渲染图像进行定性评价。结果各淋巴管CEI在5 ~ 10min达到峰值。2组患者10 min平均CEI （193.0 HU）、3组患者10 min平均CEI （2010.5 HU）显著高于1组患者（70.7 HU） （p = 0.024）。3组淋巴系统各项指标的连续性和总体诊断可接受性（分别为3.6和3.0）优于1组（分别为2.6和1.6）和2组（分别为3.0和2.6）（p = 0.249和0.204）。结论结内DCCTL的最佳成像方案为注射300 mgI/mL碘造影剂后5和10 min的双期成像。DCCTL提供了良好的淋巴管图像，在临床环境中具有潜在的可行性。
Objectives To evaluate the optimal imaging protocol and the feasibility of intranodal dynamic contrast-enhanced computed tomography lymphangiography (DCCTL) in microminipigs. Methods The Committee for Animal Research and Welfare provided university approval. Five female microminipigs underwent DCCTL after inguinal lymph node injection of 0.1 mL/kg of iodine contrast media at a rate of 0.3 mL/min with three different iodine concentrations: group 1, 75 mgI/mL; group 2, 150 mgI/mL; and group 3, 300 mgI/mL. The CT values of the venous angle, thoracic duct (TD), cisterna chyli, iliac lymphatic duct, and iliac lymph node were measured; increases in CT values pre- to post-contrast were assessed as the contrast-enhanced index (CEI). Multi-detector row CT (MDCT) and volume rendering images showing the highest CEI were qualitatively evaluated. Results The CEI of all lymphatics peaked at 5-10 min. The mean CEI of TD at 10 min of group 2 (193.0 HU) and group 3 (201.5 HU) were significantly higher than that of group 1 (70.7 HU) (p = 0.024). The continuity and overall diagnostic acceptability of all lymphatic system components were better in group 3 (3.6 and 3.0, respectively) than group 1 (2.6 and 1.6) and group 2 (3.0 and 2.6) (p = 0.249 and 0.204). Conclusions The optimal imaging protocol for intranodal DCCTL could be dual-phase imaging at 5 and 10 min after the injection of 300 mgI/mL iodinated contrast media. DCCTL provided good images of lymphatics and is potentially feasible in clinical settings.