CD71high population represents primitive erythroblasts derived from mouse embryonic stem cells
CD71high population represents primitive erythroblasts derived from mouse embryonic stem cells
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CD71high 群体代表源自小鼠胚胎干细胞的原始成红细胞
DOI：
10.1016/j.scr.2014.11.002
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发表时间：
2015-01-01
影响因子：
1.2
通讯作者：
Wang, Yuan
中科院分区：
文献类型：
--
作者：
Chao, Ruihua;Gong, Xueping;Wang, Yuan
CD 71/Ter 119组合已被广泛用于反映体内红细胞的动态成熟。然而，由于CD 71在所有增殖细胞上表达，因此尚不清楚它是否可以在胚胎干细胞（ESC）分化期间用作红细胞特异性标记物。在这项研究中，我们发现，在小鼠胚胎干细胞分化过程中表达高水平的CD 71（CD 71（高））的人口代表了在体外对应的卵黄囊来源的原始成红细胞（EryPs）在8.5天后分离cochromium。此外，这些CD 71（高）细胞经历了“成熟珠蛋白转换”，并在体外终末分化过程中去核，这与体内卵黄囊来源的EryPs相似。我们进一步证明了CD 71（高）群体的形成受到Scl，HoxB 4，Eaf 1和Klf 1等关键因子的差异调节。总之，我们的研究提供了一个技术进步，允许有效的分离EryPs从分化的胚胎干细胞在体外进一步了解原始红细胞生成过程中的分子调控。(C)2014作者由爱思唯尔公司出版
The CD71/Ter119 combination has been widely used to reflect dynamic maturation of erythrocytes in vivo. However, because CD71 is expressed on all proliferating cells, it is unclear whether it can be utilized as an erythrocyte-specific marker during differentiation of embryonic stem cells (ESCs). In this study, we revealed that a population expressing high level of CD71 (CD71(high)) during mouse ESC differentiation represented an in vitro counterpart of yolk sac-derived primitive erythroblasts (EryPs) isolated at 8.5 days post coitum. In addition, these CD71(high) cells went through "maturational globin switching" and enucleated during terminal differentiation in vitro that were similar to the yolk sac-derived EryPs in vivo. We further demonstrated that the formation of CD71(high) population was regulated differentially by key factors including Scl, HoxB4, Eaf1, and Klf1. Taken together, our study provides a technical advance that allows efficient segregation of EryPs from differentiated ESCs in vitro for further understanding molecular regulation during primitive erythropoiesis. (C) 2014 The Authors. Published by Elsevier B.V.