Injectable colloidal hydrogel with mesoporous silica nanoparticles for sustained co-release of microRNA-222 and aspirin to achieve innervated bone regeneration in rat mandibular defects
Injectable colloidal hydrogel with mesoporous silica nanoparticles for sustained co-release of microRNA-222 and aspirin to achieve innervated bone regeneration in rat mandibular defects
复制标题
具有介孔二氧化硅纳米颗粒的可注射胶体水凝胶，可持续共释放 microRNA-222 和阿司匹林，以实现大鼠下颌缺损的神经支配骨再生
DOI：
10.1039/c9tb00025a
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发表时间：
2019-04-28
影响因子：
7
通讯作者：
Chen, Xin
中科院分区：
文献类型：
--
作者：
Lei, Lei;Liu, Zhongning;Chen, Xin
神经纤维和血管在骨形成中起重要作用，骨缺损区神经支配不足可延迟再生过程。然而，很少有研究旨在促进神经支配，工程骨形成。在这里，我们报告了一种可注射的温敏性介孔二氧化硅纳米颗粒（MSN）嵌入的核-壳结构的聚（乙二醇）-b-聚（乳酸-乙醇酸）-b-聚（N-异丙基丙烯酰胺）（PEG-PLGA-PNIPAM）水凝胶的开发，用于microRNA-222和阿司匹林（ASP）的局部和长期共递送（miR 222/MSN/ASP水凝胶）。如前所述，发现ASP刺激骨形成，并且miR 222通过Wnt/β-连环蛋白/Nemo样激酶信号传导诱导人骨髓间充质干细胞分化为神经样细胞。在大鼠下颌骨缺损中，注射共递送的MSN水凝胶导致神经发生和增强的骨形成，表明本发明的可注射的miR 222-和ASP-共递送的胶体水凝胶是用于神经支配的骨组织工程的有前途的材料。
Nerve fibers and vessels play important roles in bone formation, and inadequate innervation in the bone defect area can delay the regeneration process. However, there are few studies aiming to promote innervation to engineer bone formation. Here, we report the development of an injectable thermoresponsive mesoporous silica nanoparticle (MSN)-embedded core-shell structured poly(ethylene glycol)-b-poly(lacticco-glycolic acid)-b-poly(N-isopropylacrylamide) (PEG-PLGA-PNIPAM) hydrogel for localized and long-term co-delivery of microRNA-222 and aspirin (ASP) (miR222/MSN/ASP hydrogel). ASP was found to stimulate bone formation as previously reported, and miR222 induced human bone mesenchymal stem cell differentiation into neural-like cells through Wnt/b-catenin/Nemo-like kinase signaling. In a rat mandibular bone defect, injection of the co-delivered MSN hydrogel resulted in neurogenesis and enhanced bone formation, indicating that the present injectable miR222- and ASP-co-delivering colloidal hydrogel is a promising material for innervated bone tissue engineering.