Distribution and function of splash, an achaete-scute homolog in the adult olfactory organ of the Caribbean spiny lobster Panulirus argus.
Distribution and function of splash, an achaete-scute homolog in the adult olfactory organ of the Caribbean spiny lobster Panulirus argus.
复制标题
飞溅的分布和功能，是加勒比大螯虾成年嗅觉器官中的无毛鳞片同源物。
DOI：
10.1002/dneu.20862
复制
发表时间：
2011
影响因子：
3
通讯作者：
Derby,CharlesD
中科院分区：
文献类型：
--
作者：
Tadesse,Tizeta;Schmidt,Manfred;Walthall,WilliamW;Tai,PhangC;Derby,CharlesD
achaete‐scute complex(ASC) genes, which encode basic helix‐loop‐helix transcription factors, regulate embryonic and adult neurogenesis in many animals. In adult arthropods, including crustaceans,ASChomologs have been identified but rarely functionally characterized. We took advantage of the recently identified crustacean homolog,splash(spinylobsterachaetescute homolog), in the olfactory organ of the Caribbean spiny lobsterPanulirus argusto examine its role in adult neurogenesis. We tested the hypothesis thatsplashis associated with but not restricted to sensory neuron formation in the olfactory organ, the antennular lateral flagellum (LF), of adult spiny lobsters. We demonstratedsplashlabeling in epithelial cells across LF developmental zones (i.e., proliferation and mature zones), in auxiliary cells surrounding dendrites of olfactory receptor neurons (ORNs), and in immature and mature ORNs, but not in granulocytes or chromatophores. Since ORN proliferation varies with molt stage, we examinedsplashexpression across molt stages and found that molt stage affectedsplashexpression in the ORN mature zone but not in the proliferation zone.In vivoincorporation of bromodeoxyuridine (BrdU) showed no correlation in the cellular pattern ofsplashexpression and BrdU labeling. The intensity ofsplashlabeling was dramatically enhanced in the proliferation zones following LF damage, suggesting enhancedsplashexpression during repair and/or regeneration. We conclude thatsplashis not closely associated with the formation of sensory neurons under normal physiological conditions, and we propose thatsplashis involved in repair and regeneration. We also propose thatsplashhas additional roles other than neurogenesis in adult crustaceans. © Wiley Periodicals, Inc. Develop Neurobiol 71: 316–335, 2011
