EXPLOITING THE TRIPLE RESPONSE OF ARABIDOPSIS TO IDENTIFY ETHYLENE-RELATED MUTANTS
EXPLOITING THE TRIPLE RESPONSE OF ARABIDOPSIS TO IDENTIFY ETHYLENE-RELATED MUTANTS
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DOI：
10.1105/tpc.2.6.513
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发表时间：
1990-06-01
期刊：
影响因子：
11.6
通讯作者：
ECKER, JR
中科院分区：
文献类型：
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作者：
GUZMAN, P;ECKER, JR
在黑暗中生长的幼苗乙烯的反应（“三重反应”）的变化被用来分离在拟南芥中的乙烯相关的突变体的集合。显示组成型反应（eto 1）的突变体被发现产生至少40倍以上的乙烯比野生型。在乙烯生物合成或乙烯作用被抑制的条件下，黄化eto 1 -1幼苗的形态缺陷恢复到野生型。突变体，未能显示顶端钩在乙烯（his 1）的情况下表现出减少乙烯生产。在乙烯处理下，黄化的his 1 -1幼苗下胚轴和根的伸长受到抑制，但未观察到尖钩。突变体在下胚轴和根反应中均不敏感，并显示顶端钩。每一个“三重反应”突变体对幼苗的形状和激素的产生都有影响。这些突变体应被证明是有用的工具，解剖乙烯在植物中的作用模式。
Alterations in the response of dark-grown seedlings to ethylene (the "triple response") were used to isolate a collection of ethylene-related mutants in Arabidopsis thaliana. Mutants displaying a constitutive response (eto1) were found to produce at least 40 times more ethylene than the wild type. The morphological defects in etiolated eto1-1 seedlings reverted to wild type under conditions in which ethylene biosynthesis or ethylene action were inhibited. Mutants that failed to display the apical hook in the absence of ethylene (his1) exhibited reduced ethylene production. In the presence of exogenous ethylene, hypocotyl and root of etiolated his1-1 seedlings were inhibited in elongation but no apical hook was observed. Mutants that were insensitivity in both hypocotyl and root responses, and showed an apical hook. Each of the "triple response" mutants has an effect on the shape of the seedling and on the production of the hormone. These mutants should prove to be useful tools for dissecting the mode of ethylene action in plants.