Two subsets of dendritic cells are present in human cerebrospinal fluid
Two subsets of dendritic cells are present in human cerebrospinal fluid
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DOI：
10.1093/brain/124.3.480
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发表时间：
2001-03-01
期刊：
影响因子：
14.5
通讯作者：
Link, H
中科院分区：
文献类型：
--
作者：
Pashenkov, M;Huang, YM;Link, H
人们对人类中枢神经系统中树突状细胞的存在知之甚少，为了研究脑脊液中树突状细胞的存在，使用流式细胞术检查了多发性硬化症、急性视神经炎、莱姆神经疏螺旋体病、其他炎症性神经系统疾病和非炎症性神经系统疾病患者的配对血液/脑脊液样本，几乎所有脑脊液样本都含有髓样细胞（lin(-)CD11c+ HLA-DR(++)CD123(dim)）和浆细胞样 (lin-CD11c(+)HLA-DR(+)CD123(高)) 树突状细胞。在非炎症性神经系统疾病中，任一亚群的树突状细胞仅占脑脊液单核细胞的 1%。髓样脑脊液树突状细胞在视神经炎、神经疏螺旋体病和其他炎症性神经系统疾病中升高，而浆细胞样树突状细胞在所有研究的神经炎症性疾病中均升高，其中神经疏螺旋体病的数量尤其高。脑脊液树突状细胞的数量与中枢神经系统炎症的常见参数相关。脑脊液中的髓系树突状细胞比血液中表达更高水平的HLA-DR、CD86、CD80和CD40，而浆细胞样树突状细胞在血液和脑脊液中表达这些分子是相等的。脑脊液和血液树突状细胞都表达趋化因子受体CCR5。这是树突状细胞存在于人脑脊液中并且浆细胞样树突状细胞存在于非淋巴室、髓系和脑脊液中的首次证明。脑脊液中的浆细胞样树突状细胞可能有助于协调局部免疫反应。
Little is known about the presence of dendritic cells in the human CNS, To investigate the occurrence of dendritic cells in the CSF, paired blood/CSF samples from patients with multiple sclerosis, acute optic neuritis, Lyme neuroborreliosis, other inflammatory neurological diseases and non-inflammatory neurological diseases were examined using flow cytometry, Almost all CSF samples contained myeloid (lin(-)CD11c+ HLA-DR(++)CD123(dim)) and plasmacytoid (lin-CD11c(+)HLA-DR(+)CD123(high)) dendritic cells. In non-inflammatory neurological diseases, dendritic cells of either subset only constituted up to 1% of CSF mononuclear cells. Myeloid CSF dendritic cells were elevated in optic neuritis, neuroborreliosis and other inflammatory neurological disorders, while plasmacytoid dendritic cells were elevated in all neuroinflammatory conditions studied, with especially high numbers in neuroborreliosis. Numbers of CSF dendritic cells correlated with the common parameters of CNS inflammation. The myeloid dendritic cells in CSF expressed higher levels of HLA-DR, CD86, CD80 and CD40 than those in blood, whereas expression of these molecules by plasmacytoid dendritic cells was equal in blood and CSF, Both CSF and blood dendritic cells expressed the chemokine receptor CCR5, This is the first demonstration that dendritic cells are present in human CSF and that plasmacytoid dendritic cells are present in a nonlymphoid compartment, Myeloid and plasmacytoid dendritic cells in CSF may contribute to orchestration of the local immune responses.