CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression
CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression
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DOI：
10.1038/nbt1399
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发表时间：
2008-05-01
影响因子：
46.9
通讯作者：
Gill, Deborah R.
中科院分区：
文献类型：
--
作者：
Hyde, Stephen C.;Pringle, Ian A.;Gill, Deborah R.
质粒DNA (pDNA)/阳离子脂质体复合物的肺传递与急性未甲基化CG二核苷酸（CpG）介导的炎症反应和转基因表达的短暂持续时间有关。我们证明，即使在pDNA中保留单个CpG也足以引发炎症反应，而无CpG的pDNA载体则不会。使用不含cpg的pDNA表达载体，我们在没有肺部炎症的情况下实现了持续（>= 56 d）的体内转基因表达。
Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (>= 56 d) in vivo transgene expression in the absence of lung inflammation.