New technologies for monitoring human antigen-specific T cells and regulatory T cells by flow-cytometry
New technologies for monitoring human antigen-specific T cells and regulatory T cells by flow-cytometry
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DOI：
10.1016/j.coph.2015.04.005
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发表时间：
2015-08-01
影响因子：
4
通讯作者：
Scheffold, Alexander
中科院分区：
文献类型：
--
作者：
Bacher, Petra;Scheffold, Alexander
T 细胞协调并执行针对其抗原受体识别的某些抗原的免疫反应。它们可以获得高度多样化的功能特性，为免疫保护和免疫病理学提供基础，从而代表高度特异性的诊断和治疗靶点。新的细胞计数技术现在可以识别和精确表征针对基本上任何抗原的人类常规和调节性 T 细胞，甚至是天然供体中的 T 细胞。这些为针对感染的免疫保护的彻底分析和解决未满足的挑战提供了基础，例如涉及耐受性和/或针对未定义或复杂抗原（即自身免疫或过敏）的T细胞反应。连同单细胞多参数方法的并行发展，这彻底改变了人类 T 细胞的定量和定性特征，具有重要的诊断、预后或治疗潜力。
T cells orchestrate and execute immune responses against certain antigens recognized by their antigen receptor. They can acquire a highly divers set of functional properties, which provide the basis for immune protection, but also for immune-pathologies and thus represent highly specific diagnostic and therapeutic targets. New cytometric technologies now allow identification and precise characterization of human conventional and regulatory T cells against basically any antigen and even within naive donors. These provide the basis for thorough analyses of immune protection against infections and to tackle unmet challenges such as T cell responses involved in tolerance and/or directed against undefined or complex antigens, that is, in autoimmunity or allergy. Together with the parallel evolution of single cell multi-parameter approaches this has revolutionized the quantitative and qualitative characterization of human T cells, bearing important diagnostic, prognostic or therapeutic potential.