OSTEOGENIC PROTEIN-1 INDUCES DENDRITIC GROWTH IN RAT SYMPATHETIC NEURONS
OSTEOGENIC PROTEIN-1 INDUCES DENDRITIC GROWTH IN RAT SYMPATHETIC NEURONS
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DOI：
10.1016/0896-6273(95)90148-5
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发表时间：
1995-09-01
期刊：
影响因子：
16.2
通讯作者：
HIGGINS, D
中科院分区：
文献类型：
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作者：
LEIN, P;JOHNSON, M;HIGGINS, D
从围产期大鼠幼崽的交感神经元延长只有一个单一的轴突时，保持在文化中的神经胶质细胞和血清的情况下。暴露于重组成骨蛋白-1（OP-1）选择性地诱导树突的形成，所述树突正确地分离和修饰细胞骨架和膜蛋白并形成适当极性的突触接触。OP-1需要神经生长因子（NGF）作为辅助因子，并且，在最佳浓度的NGF的存在下，OP-1诱导的培养的围产期神经元的树突状生长与原位观察到的相当。在原位没有形成树突的交感神经母细胞也对培养物中的OP-1有反应，表明OP-1可以引起树突的从头形成以及再生。这些数据表明，特定的信号可以调节神经元的形状和极性的发展。
Sympathetic neurons from perinatal rat pups extend only a single axon when maintained in culture in the absence of glia and serum. Exposure to recombinant osteogenic protein-1 (OP-1) selectively induces the formation of dendrites that correctly segregate and modify cytoskeletal and membrane proteins and form synaptic contacts of appropriate polarity. OP-1 requires nerve growth factor (NGF) as a cofactor, and, in the presence of optimal concentrations of NGF, OP-1-induced dendritic growth from cultured perinatal neurons is comparable to that observed in situ. Sympathetic neuroblasts that had not formed dendrites in situ also responded to OP-1 in culture, indicating that OP-l can cause de novo formation as well as regeneration of dendrites. These data imply that specific signals can regulate the development of neuronal shape and polarity.