Gastric antisecretory effect of serotonin: quantitation of release and site of action.
Gastric antisecretory effect of serotonin: quantitation of release and site of action.
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血清素的胃抗分泌作用：释放定量和作用部位。
DOI：
10.1152/ajpendo.1996.271.4.e669
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
StephensJr,RL
中科院分区：
文献类型：
--
作者：
Lepard,KJ;Chi,J;Mohammed,JR;Gidener,S;StephensJr,RL
尽管许多报道表明血清素 (5-HT) 抑制胃酸排出，但内源性 5-HT 调节胃分泌的作用和作用机制仍不清楚。迷走神经刺激增强了大鼠胃腔（600%）和门静脉循环（265%）的 5-HT 释放的基础速率。 5-HT 释放到门脉循环的峰值速率比腔内释放高 1,000 倍（分别为 12 微克/分钟和 1.2 纳克/分钟）。为了阐明 5-HT 抑制酸分泌的作用位点，采取了几种方法。腔内灌注外源性 5-HT 以提高迷走神经刺激后的水平，但不会减少胃酸排出量。相比之下，全身注射 5-HT 可将门静脉 5-HT 升高至与迷走神经刺激相似的水平，具有显着的抗分泌作用。化学或手术消融支配胃的肠神经或交感神经并不会减弱外源性 5-HT 对胃酸排出的抑制作用。全身5-HT的抗分泌作用对吡罗昔康、多沙曲唑、胃动脉内硝普钠、生长抑素单克隆抗体或双侧肾上腺切除术预处理不敏感。结果表明，在迷走神经刺激后，5-HT从内源性储存释放到门静脉循环中，以改变胃的生理机能，并且其作用与自主神经系统、胃粘膜前列腺素或生长抑素、粘膜肥大细胞或肾上腺成分或胃粘膜血流的变化无关。
Despite many reports that serotonin (5-HT) inhibits gastric acid output, the role and mechanism of action of endogenous 5-HT to modulate gastric secretion remain unclear. Vagal stimulation enhanced the basal rate of 5-HT release into both the gastric lumen (600%) and the portal circulation (265%) of the rat. The peak rate of 5-HT release into the portal circulation was 1,000-fold higher that luminal release (12 micrograms/min and 1.2 ng/min, respectively). To elucidate site(s) of action of 5-HT to inhibit acid secretion, several approaches were taken. Intraluminal perfusion of exogenous 5-HT to encompass enhanced levels seen after vagal stimulation did not reduce gastric acid output. In contrast, administration of systemic 5-HT, which raised portal venous 5-HT to similar levels as vagal stimulation, had a marked antisecretory effect. Chemical or surgical ablation of enteric or sympathetic nerves innervating the stomach did not attenuate the inhibitory effect of exogenous 5-HT on gastric acid output. The antisecretory effect of systemic 5-HT was insensitive to pretreatment with piroxicam, doxantrazole, close gastric intra-arterial sodium nitroprusside, somatostatin monoclonal antibody, or bilateral adrenalectomy. The results suggest that 5-HT is released from endogenous stores into the portal circulation in sufficient quantities after vagal stimulation to alter gastric physiology and that its action is independent of the autonomic nervous system, gastric mucosal prostaglandins or somatostatin, mucosal mast cell or adrenal constituents, or changes in gastric mucosal blood flow.