Precise excision of TTAA-specific lepidopteran transposons piggyBac(IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera
Precise excision of TTAA-specific lepidopteran transposons piggyBac(IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera
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DOI：
10.1111/j.1365-2583.1996.tb00048.x
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发表时间：
1996-05-01
影响因子：
2.6
通讯作者：
Bauser, C
中科院分区：
文献类型：
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作者：
Fraser, MJ;Coszczon, T;Bauser, C
杆状病毒的转座子突变为分析鳞翅目基因组中一个独特的可移动元件家族的运动提供了一个理想的实验系统，该家族的成员对TTAA靶点具有极强的特异性。本报告描述了该家族的两个代表，tagalong（以前的TFP3）和piggyBac（以前的IFP2）的切除事件分析。用多面蛋白/lacZ报告基因标记这些元件，并通过同源重组或转位插入病毒基因组中，根据白斑表型选择复归物。在n -368细胞或IPLB-SF21 AE细胞中，这两个元件都以精确的方式从它们在杆状病毒基因组中的位置切除，在感染的IPLB-SF21 AE细胞中，这些元件的精确切除发生在缺乏tagalong或piggySac元件编码功能的情况下。这些元件的插入和切除的共同特征进一步证实了它们包含在一个独特的转座子家族中。
Transposon mutagenesis of baculoviruses provides an ideal experimental system for analysis of the movement of a unique family of mobile element identified from lepidopteran genomes, Members of this family of short-inverted-repeat elements are characterized by their extreme specificity for TTAA target sites. This report describes the analysis of excision events for two representatives of this family, tagalong (formerly TFP3) and piggyBac (formerly IFP2). These elements were tagged with a polyhedrin/lacZ reporter gene and inserted back into the virus genome either by homologous recombination or by transposition, Revertants were selected based on a white plaque phenotype. Both elements excise in a precise fashion from their positions in the baculovirus genome in either TN-368 cells or IPLB-SF21 AE cells, The precise excision of these elements in infected IPLB-SF21 AE cells occurs in the absence of either tagalong or piggySac element encoded functions. The common characteristics of both insertion and excision for these elements provides further validation for their inclusion in a single family of unique transposons.