Chemoprevention by lipid-soluble tea polyphenols in diethylnitrosamine/phenobarbital-induced hepatic pre-cancerous lesions.
Chemoprevention by lipid-soluble tea polyphenols in diethylnitrosamine/phenobarbital-induced hepatic pre-cancerous lesions.
复制标题
脂溶性茶多酚对二乙基亚硝胺/苯巴比妥诱导的肝癌前病变的化学预防作用。
作者：
DE Stefano, Jamie;Yang, Jun;Xu, Li-Hong;Zhu, Xin-Qiang
背景
绿色茶多酚（GTPs）是一种有希望的化学预防剂。然而，GTP水平在血液中保持相对较低，并且化学不稳定。脂溶性茶多酚（LTP）是由脂肪酸通过酯键对GTP进行改性的产物。LTP是亲脂性的，并且与水溶性多酚相比，预期在体内提供改善的吸收和利用。本研究旨在探讨LTP对二乙基亚硝胺（DEN）诱发的大鼠肝癌的化学预防作用及其可能机制。
材料和方法
在DEN注射前2周开始以0、40和400 mg/kg/天的剂量经口给予LTP，并持续30周。在该时间点采集样品并进行肝脏组织病理学分析。
结果
LTP减少了DEN治疗大鼠肝脏样本中胎盘谷胱甘肽S-转移酶阳性病灶的面积和数量。此外，LTP抵消了DEN诱导的肝纤维化形成。大鼠肝脏的免疫组化染色显示，LTP抑制DEN介导的PCNA和8-OHdG阳性细胞数量的升高。
结论
本研究首次证明，LTP通过抑制大鼠肝脏模型中的细胞增殖和DNA损伤，对癌前病变发挥化学预防作用。
BACKGROUND
Green tea polyphenols (GTPs) have been proposed as promising candidates for chemoprevention. However, GTPs levels are maintained relatively low in the blood and are chemically-unstable. Lipid-soluble tea polyphenols (LTPs) are products of modified GTPs with ester linkage with fatty acids. LTPs are lipophilic and expected to provide improved absorption and utilization in the body compared with water-soluble polyphenols. The current study was designed to investigate the chemo-preventive property and the possible mechanisms of LTP action against diethylnitrosamine (DEN)-induced liver cancer in rats.
MATERIALS AND METHODS
Oral administration of LTPs at doses of 0, 40, and 400 mg/kg/day was initiated 2 weeks prior to DEN injection and was continued for 30 weeks. At that time point samples were collected and liver histopathological analyses were performed.
RESULTS
LTPs decreased the area and number of placental glutathione S-transferase-positive foci in liver samples of DEN-treated rats. Furthermore, LTPs counteracted DEN-induced fibrosis formation in liver. Immunohistochemical staining of rat liver showed that LTPs inhibited DEN-mediated elevations in numbers of cells positive for PCNA and 8-OHdG.
CONCLUSION
For the first time, the present study demonstrated, that LTPs exert a chemo-preventive effect against precancerous lesions through inhibition of cellular proliferation and DNA damage in a rat liver model.
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影响因子：
81.5
作者：
Llovet, Josep M.;Zucman-Rossi, Jessica;Gores, Gregory
通讯作者：
Gores, Gregory
DOI：
10.1111/j.1346-8138.2000.tb02259.x
发表时间：
2000-11
期刊：
The Journal of Dermatology
影响因子：
--
作者：
D. Bickers;M. Athar
通讯作者：
D. Bickers;M. Athar
肝癌发生过程中与肝细胞结节中肝细胞抗性相关的独特生化模式。
影响因子：
10.4
作者：
通讯作者：
--
影响因子：
5.1
作者：
Marrero, Jorge A;Pelletier, Shawn
通讯作者：
Pelletier, Shawn
影响因子：
1.5
作者：
Gilles, HoChong;Garbutt, Tonora;Landrum, Jasmine
通讯作者：
Landrum, Jasmine