Fitness cost of insecticide resistance on the life-traits of a Anopheles coluzzii population from the city of Yaoundé, Cameroon.
Fitness cost of insecticide resistance on the life-traits of a Anopheles coluzzii population from the city of Yaoundé, Cameroon.
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抗杀虫剂的适应性成本在喀麦隆Yaoundé市的Anopheles Coluzzii人口的生命特征上。
DOI：
10.12688/wellcomeopenres.16039.2
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发表时间：
2020
影响因子：
--
通讯作者：
Antonio-Nkondjio C
中科院分区：
文献类型：
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作者：
Nkahe DL;Kopya E;Djiappi-Tchamen B;Toussile W;Sonhafouo-Chiana N;Kekeunou S;Mimpfoundi R;Awono-Ambene P;Wondji CS;Antonio-Nkondjio C
背景：拟除虫菊酯耐药性正在迅速扩大。冈比亚有限公司撒哈拉以南非洲地区的人口。然而，关于杀虫剂抗药性的适应成本仍然没有足够的信息。在本研究中，调查了雅温得市的按蚊种群对杀虫剂抗性的适应成本。 方法：A抗性An。 coluzzii 群体是从现场采集的对 DDT 和拟除虫菊酯均具有抗性的蚊子中建立的，并用 0.05% 溴氰菊酯筛选了 12 代。 Ngousso 实验室敏感菌株用作对照。每个品系共100只雌性进行血液喂养并允许单独产卵，然后测量不同的生命性状参数，如繁殖力、生育力、幼虫发育时间、羽化率和寿命。 TaqMan 测定用于筛选 L1014F 和 L1014S kdr 突变的存在。 结果：现场采集的 F0 代蚊子，滴滴涕的死亡率为 2.05%，氯菊酯为 34.16%，溴氰菊酯为 50.23%。 F12代的死亡率对于溴氰菊酯为30.48%，对于氯菊酯为1.25%，对于滴滴涕为0%。与易感群体相比，抗性群体中每只雌性产卵的数量较低（p <0.0001）。与易感幼虫（7.57±0.35 天）相比，抗杀虫剂幼虫的幼虫发育时间（10.61±0.33 天）显着较长。与抗性组相比，易感组中新生雌性的数量明显较高。与耐药性（14.63±0.68 天）相比，易感性成虫的寿命（21.73±1.19 天）也显着较高。在抗性群体中仅检测到 L1014F-kdr 等位基因。结论：研究表明，拟除虫菊酯抗性可能与按蚊群体的高适应度成本相关。添加专门针对幼虫阶段的新工具可以改善疟疾媒介控制和杀虫剂抗性管理。
Background: Pyrethroid resistance is rapidly expanding in An. gambiae s.l. populations across Sub-Saharan Africa. Yet there is still not enough information on the fitness cost of insecticide resistance . In the present study, the fitness cost of insecticide resistance on Anopheles coluzzii population from the city of Yaoundé was investigated. Methods: A resistant An. coluzzii colony was established from field collected mosquitoes resistant to both DDT and pyrethroid and selected for 12 generations with deltamethrin 0.05%. The Ngousso laboratory susceptible strain was used as control. A total of 100 females of each strain were blood fed and allowed for individual eggs laying, and then different life traits parameters such as fecundity, fertility, larval development time, emergence rate and longevity were measured. The TaqMan assay was used to screen for the presence of the L1014F and L1014S kdr mutations. Results: Field collected mosquitoes from the F0 generation had a mortality rate of 2.05% for DDT, 34.16% for permethrin and 50.23% for deltamethrin. The mortality rate of the F12 generation was 30.48% for deltamethrin, 1.25% for permethrin and 0% for DDT. The number of eggs laid per female was lower in the resistant colony compared to the susceptible (p <0.0001). Insecticide resistant larvae were found with a significantly long larval development time (10.61±0.33 days) compare to susceptible (7.57±0.35 days). The number of emerging females was significantly high in the susceptible group compared to the resistant . The adults lifespan was also significantly high for susceptible (21.73±1.19 days) compared to resistant (14.63±0.68 days). Only the L1014F- kdr allele was detected in resistant population.. Conclusion: The study suggests that pyrethroid resistance is likely associated with a high fitness cost on An.coluzzii populations. The addition of new tools targeting specifically larval stages could improve malaria vectors control and insecticide resistance management.