An Efficient Protocol for Callus Induction in Aquilaria malaccensis Lam. Using Leaf Explants at Varied Concentrations of Sucrose
An Efficient Protocol for Callus Induction in Aquilaria malaccensis Lam. Using Leaf Explants at Varied Concentrations of Sucrose
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一种有效的沉香愈伤组织诱导方案。
DOI：
10.5923/j.plant.20120206.03
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
M. S. Singh
中科院分区：
文献类型：
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作者：
M. Saikia;K. Shrivastava;S. Sureshkumar;M. S. Singh
白木香（科：Thy melaeaceae），通常被称为沉香，鹰木或Gaharu是印度东北部的一种商业上重要的树种。沉香树在印度被列为极度濒危物种。本种由于其珍贵的心材而被持续开发，心材是用于生产高级香水以及传统药物的昂贵琼脂油的来源。本研究的主要目的是建立一个快速的愈伤组织产生方案，可以作为一个重要的选择，直接提取琼脂油。为此，需要大规模生产愈伤组织。通过叶片的增殖培养，建立了一套有效的马来沉香愈伤组织再生体系。用Murashige和Skoog培养基，在不同浓度的蔗糖（2-5%）下，添加最少量的植物生长激素（2，4-D，NAA，Kinetin和BAP）。各种激素和糖浓度处理均能诱导出愈伤组织，但以MS + BAP（0.5mg/l）+ NAA（3 mg/l）培养基的愈伤组织生长最好，在最适蔗糖浓度（4%）下培养45-60天，细胞鲜重（7.368g）和干重（2.170g）最高。
Aquilaria malaccensis Lam. (Family: Thy melaeaceae), co mmonly known as agarwood, eagle wood or Gaharu is a co mmercially important tree species of northeast India. The Aquilaria tree is categorised as critically endangered in Ind ia. This species is being continuously exploited due to its precious heartwood which is the source of expensive agar oil used in the production of high grade perfu mes as well as in trad itional medicines. The main aim of this study was to establish a speedy callus production protocol fro m Aquilaria malaccensis which may serve as an important option for direct extraction of agar o il. For this purpose, large scale p roduction of callus tissue is needed. An efficient callus regeneration protocol was established through leaf pro liferation in Aquilaria malaccensis Lam. using Murashige and Skoog mediu m supplemented with least amount of plant growth hormones (2,4 D, NAA, Kinetin & BAP) at varied concentrations of sucrose (2-5%). Callus could be initiated in all the treat ments of hormones and sugar levels; however, the best callus growth was obtained in the MS med iu m supplemented with BAP (0.5 mg/l) + NAA (3mg/l) g iving the h ighest fresh (7.368g) and dry cell b io mass (2.170g) at the optimu m sucrose concentration (4%) after 45-60 days of incubation.