Esterase activity inspired selection and characterization of zearalenone degrading bacteria Bacillus pumilus ES-21
Esterase activity inspired selection and characterization of zearalenone degrading bacteria Bacillus pumilus ES-21
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酯酶活性启发玉米赤霉烯酮降解菌短小芽孢杆菌 ES-21 的选择和表征
DOI：
10.1016/j.foodcont.2017.01.021
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发表时间：
2017-07-01
期刊：
影响因子：
6
通讯作者：
Xu, Jianhong
中科院分区：
文献类型：
--
作者：
Wang, Gang;Yu, Mingzheng;Xu, Jianhong
玉米赤霉烯酮（ZEN）是一种主要由镰刀菌属真菌产生的雌激素类真菌毒素，可引起家畜的生殖障碍。在这项研究中，我们描述了一种通过酯酶活性测定筛选ZEN降解细菌的简单快速方法。首先测试土壤细菌菌株的酯酶活性，然后进一步评估活性菌株的ZEN降解潜力。一种名为短小芽孢杆菌（Bacillus pumilus）ES-21的细菌菌株被检测到能够消除培养基中的ZEN。通过响应面法优化了ZEN的降解条件，结果表明，当ZEN浓度为17.9 g/ml时，短小芽孢杆菌ES-21在24 h内对ZEN的降解率可达95.7%。根据LC-TOF-MS/MS分析，其中一种降解产物拟定为1-（3，5-二羟基苯基）-6 '-羟基-F-十一烯-10'-酮。本研究为ZEN降解菌的筛选提供了一种新的方法，并为ZEN降解菌的筛选提供了一种新的途径。(C)2017爱思唯尔有限公司版权所有
Zearalenone (ZEN), mainly produced by Fusarium species, is an estrogenic mycotoxin which causes reproductive disorders in livestock. In this study, we described a simple and rapid method for screening of ZEN-degrading bacteria by esterase activity assay. Soil bacteria strains were first tested for their esterase activities, then active strains were further evaluated for their ZEN-degrading potentials. A bacterial strain named Bacillus pumilus ES-21 was detected to be able to eliminate ZEN in the culture medium. ZEN degradation conditions were optimized through response surface methodology and the result showed that the degradation rate of ZEN by Bacillus pumilus ES-21 was up to 95.7% at the ZEN concentration of 17.9 g/ml within 24 h. One of the degradation product was proposed to be 1-(3,5-ihydroxypheny1)-6'-hydroxy-F-undecen-10'-one according to LC-TOF-MS/MS analysis. This study provided a strategy for the isolation of ZEN degrading microbes and a promising degrading strain. (C) 2017 Elsevier Ltd. All rights reserved.