Humoral immune responses to phocine herpesvirus-1 in Pacific harbor seals (Phoca vitulina richardsii) during an outbreak of clinical disease
Humoral immune responses to phocine herpesvirus-1 in Pacific harbor seals (Phoca vitulina richardsii) during an outbreak of clinical disease
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DOI：
10.1016/s0378-1135(00)00369-2
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发表时间：
2001-05-03
影响因子：
3.3
通讯作者：
Stott, JL
中科院分区：
文献类型：
--
作者：
King, DP;Lie, AR;Stott, JL
1 型海豹疱疹病毒 (PHV-1) 感染与新生斑海豹 (Phoca vitulina) 的发病率和高死亡率有关。开发了PHV-1特异性间接酶联免疫吸附测定（ELISA）来连续测量太平洋海岸康复中心收治的106只斑海豹新生儿（静息血清总数为371份）的血清学状态。在季节初期（2月至4月），大多数幼崽血清中PHV-1特异性抗体水平较低。在 5 月份的 4 周内，观察到大多数住院幼犬的 PHV-1 特异性抗体急剧增加。这与 PHV-1 相关的肾上腺病变和死亡率的高发生率相一致。尽管血清转化为 PHV-1 的时间与 PHV-1 感染的组织学证据总体一致，但 82.4% 的肾上腺炎幼犬在死亡时没有对 PHV-1 体液反应的证据。这表明某些新生海豹要么病程快速，要么无法产生体液反应。 (C) 2001 Elsevier Science B.V. 保留所有权利。
Infection with phocine herpesvirus type-1 (PHV-1) has been associated with morbidity and high mortality in neonatal harbor seals (Phoca vitulina). A PHV-1 specific indirect enzyme linked immunosorbent assay (ELISA) was developed to sequentially measure the serological status of 106 harbor seal neonates admitted to a Pacific coast rehabilitation center (total number of sera rested was 371), Early in the season (February-April), the majority of pups had low serum levels of PHV-1 specific antibody. A dramatic increase in PHV-1 specific antibody, involving the majority of hospitalized pups, was observed during a 4-week period in May. This coincided with a high incidence of PHV-1 associated adrenal lesions and mortality. Although there was overall agreement between the timing of seroconversion to PHV-1 and histological evidence of PHV-1 infection, 82.4% of individual pups with adrenalitis had no evidence of a humoral response to PHV-1 at the time of their death. This suggests either a rapid disease course, or an inability to develop a humoral response in some neonatal seals. (C) 2001 Elsevier Science B.V. All rights reserved.