Genetic Modification of T Cells.
Genetic Modification of T Cells.
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DOI：
10.3390/biomedicines4020009
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发表时间：
2016-04-20
期刊：
影响因子：
4.7
通讯作者：
Boyerinas B
中科院分区：
文献类型：
--
作者：
Morgan RA;Boyerinas B
基因转移技术及其在人类基因治疗中的应用在过去十年中得到了极大的发展。一个似乎特别有希望的研究领域是将新的遗传物质转移到T细胞中，用于潜在的癌症治疗。在本文中，我们描述了几种核心技术，这些技术现在可以高效地将基因转移到原代人类T细胞中。这些基因转移技术包括基于修饰的逆转录病毒科的基于病毒的基因转移方法和非病毒方法，例如基于DNA的转座子和通过电穿孔直接转移mRNA。在引用具体实例的情况下，我们强调嵌合抗原受体（汽车）向T细胞的转移，这允许工程化T细胞识别潜在的肿瘤抗原。
Gene transfer technology and its application to human gene therapy greatly expanded in the last decade. One area of investigation that appears particularly promising is the transfer of new genetic material into T cells for the potential treatment of cancer. Herein, we describe several core technologies that now yield high-efficiency gene transfer into primary human T cells. These gene transfer techniques include viral-based gene transfer methods based on modified Retroviridae and non-viral methods such as DNA-based transposons and direct transfer of mRNA by electroporation. Where specific examples are cited, we emphasize the transfer of chimeric antigen receptors (CARs) to T cells, which permits engineered T cells to recognize potential tumor antigens.