Human immunodeficiency virus type 1 infection of cells and tissues from the upper and lower human female reproductive tract
Human immunodeficiency virus type 1 infection of cells and tissues from the upper and lower human female reproductive tract
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DOI：
10.1128/jvi.71.5.3498-3506.1997
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发表时间：
1997-05-01
影响因子：
5.4
通讯作者：
Wira, CR
中科院分区：
文献类型：
--
作者：
Howell, AL;Edkins, RD;Wira, CR
检查人输卵管、子宫、宫颈和阴道粘膜的活组织切片和分离细胞培养物对人类免疫缺陷病毒1型（HIV-1）感染的敏感性。我们使用单核细胞嗜性株HIV-1（JR-FL）和从感染新生儿中获得的几种HIV-1原代分离株检测感染性，通过短期培养中p24的产生和通过激光扫描共聚焦显微镜对HIV-1 Nef和p24蛋白的免疫荧光检测来测量HIV-1感染，使用三色免疫荧光对来自每个部位的组织切片中的HIV感染细胞进行表型分析，我们的研究结果表明，上皮细胞、基质细胞和树突状细胞以及来自女性生殖道的具有CD 14（+）CD 4（+）、CD 14（-）CD 4（-）和CD 4（+）CD 14（-）表型的细胞可感染HIV-1。重要的是发现上生殖道的组织容易感染HIV-1，此外，来自月经周期所有阶段的妇女，包括绝经后妇女的组织样本，女性生殖道细胞需要暴露于HIV-1至少60分钟才能发生感染，与外周血淋巴细胞相反，这些发现表明HIV-1可以感染来自女性生殖道内不同部位的细胞和组织，并表明多种细胞类型，包括上皮细胞，可能是HIV-1初始感染的靶点。
Viable tissue sections and isolated cell cultures from the human fallopian tube, uterus, cervix, and vaginal mucosa were examined for susceptibility to infection with human immunodeficiency virus type 1 (HIV-1). We examined infectivity by using the monocytotropic strain HIV-1(JR-FL) and several primary isolates of HIV-1 obtained from infected neonates, HIV-1 infection was measured by p24 production in short-term culture and by immunofluorescence detection of HIV-1 Nef and p24 proteins by laser scanning confocal microscopy, Three-color immunofluorescence was used to phenotype HIV-infected cells within tissue sections from each site, Our findings indicate that epithelial, stromal, and dendritic cells and cells with CD14(+) CD4(+), CD14(-)CD4(-), and CD4(+) CD14(-) phenotypes from the female reproductive tract are infectable with HIV-1. Of importance is the finding that tissues from the upper reproductive tract are susceptible to infection with HIV-1, Moreover, tissue samples from women in all stages of the menstrual cycle, including postmenopausal women (inactive), could be infected with HIV-1, Female reproductive tract cells required a minimum of 60 min of exposure to HIV-1 in order for infection to occur, in contrast to peripheral blood lymphocytes, which became infected after being exposed to HIV-1 for only 1 min, These findings demonstrate that HIV-1 can infect cells and tissues from different sites within the female reproductive tract and suggest that multiple cell types, including epithelial cells, may be targets for the initial infection by HIV-1.