A novel jasmonic acid-inducible rice myb gene associates with fungal infection and host cell death
A novel jasmonic acid-inducible rice myb gene associates with fungal infection and host cell death
复制标题
DOI：
10.1094/mpmi.2001.14.4.527
复制
发表时间：
2001-04-01
影响因子：
3.5
通讯作者：
Yang, YO
中科院分区：
文献类型：
--
作者：
Lee, MW;Qi, M;Yang, YO
茉莉酸（JA）和水杨酸（SA）等内源信号分子在诱导水稻对病原菌侵染和昆虫取食的抗性中起重要作用，JA是水稻幼苗对稻瘟病菌侵染的系统获得抗性（SAR）的有效诱导物为了进一步深入了解JA介导的防御信号通路，我们分离并鉴定了病原体和JA诱导的水稻基因（JAmyb），其编码Myb转录因子。JAmyb基因在真菌感染后1天内在病斑形成前的抗性和敏感性相互作用中被诱导。与大多数防御相关基因在抗性相互作用中被更快更强地激活不同，在易感相互作用中，由稻瘟菌诱导的JAmyb要高得多，伴随着大的病变和广泛的组织损伤。在某些病变模拟突变体中，在细胞死亡和病变形成期间也观察到JAmyb的显著诱导。有趣的是，JAmyb被JA或创伤快速激活，独立于从头蛋白质合成，但不被其他内源性信号分子如SA和脱落酸或SAR诱导剂如苯并噻二唑和烯丙苯噻唑激活。我们使用SA-缺陷的转基因植物，以进一步证明，SA在水稻中的耗尽并没有废除，而是增强了爆炸诱导的JAmyb的表达。这些结果表明，JAmyb是密切相关的寄主细胞死亡，并参与了JA介导的，SA非依赖性的信号转导途径在水稻。
Endogenous signal molecules such as jasmonic acid (JA) and salicylic acid (SA) play an important role in induced resistance against pathogen infection and insect herbivory, In rice seedlings, JA is an effective inducer of systemic acquired resistance (SAR) against infection of blast fungus (Pyricularia grisea), To gain further insights into JA-mediated defense signaling pathways, we isolated and characterized a pathogen- and JA-induced rice gene (JAmyb) that encodes a Myb transcription factor. The JAmyb gene was induced within 1 day after fungal infection in resistant and susceptible interactions prior to lesion formation. Unlike most defense-related genes that are activated faster and stronger in resistant interactions, JAmyb induction by blast fungus is much higher in susceptible interactions, accompanied by large lesions and extensive tissue damage. Significant induction of JAmyb also was observed during cell death and lesion formation in certain lesion mimic mutants. Interestingly, JAmyb was activated rapidly by JA or wounding, independent of de novo protein synthesis, but not by other endogenous signal molecules such as SA and abscisic acid or SAR inducers such as benzothiadiazole and probenazole. We used SA-deficient transgenic plants to further demonstrate that depletion of SA in rice did not abolish but rather enhanced blast-induced JAmyb expression. These results suggest that JAmyb is related closely to host cell death and is involved in the JA-mediated, SA-independent signaling pathways in rice.