Detection of fimbriae and fimbrial antigens on the oral anaerobe Bacteroides gingivalis by negative staining and serological methods.
Detection of fimbriae and fimbrial antigens on the oral anaerobe Bacteroides gingivalis by negative staining and serological methods.
复制标题
采用负染色和血清学方法检测口腔厌氧菌牙龈拟杆菌的菌毛和菌毛抗原。
DOI：
10.1099/00221287-134-10-2713
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
T. Suzuki
中科院分区：
文献类型：
--
作者：
Y. Suzuki;F. Yoshimura;K. Takahashi;H. Tani;T. Suzuki
我们用阴性染色和免疫学方法对8个不同实验室的63株牙龈类杆菌临床分离株进行了菌毛检测。所采用的技术是细菌凝集、Ouchterlone免疫扩散和Western免疫印迹分析，使用兔抗菌毛和抗菌毛的抗fimbrin血清和抗B.givalis 381菌毛的菌毛蛋白fimbrin。在51株受试菌株中，49株菌毛染色阴性，其中30株菌毛在三种免疫学检测中均呈阳性(60%)。免疫印迹分析共检出37株(75%)阳性，这是本研究中使用的最可靠的血清学方法。研究表明，大多数牙龈假单胞菌菌株都是菌毛，这些菌毛与381菌株的菌毛在免疫上是相关的。通过对不同菌株进行比较，免疫印迹分析发现fimbrin具有分子异质性。与大多数菌株，包括381菌株，抗纤毛膜蛋白血清与一个表观分子量为43 kDa的蛋白反应，而与15个菌株的免疫反应蛋白表观分子量为46 kDa。
We screened 63 clinical isolates of Bacteroides gingivalis from eight different laboratories for the presence of fimbriae by negative staining and by immunological methods. Techniques used were bacterial agglutination, Ouchterlony immunodiffusion and Western immunoblotting analysis using rabbit anti-fimbriae and anti-fimbrilin sera raised against fimbriae and fimbrilin (a constituent protein of B. gingivalis fimbriae) from B. gingivalis strain 381. In 49 of the 51 strains tested, fimbriae were clearly detected by negative staining, and 30 (60%) of the fimbriate strains were positive in all three of the immunological assays. A total of 37 strains (75%) were positive by immunoblotting analysis, which was the most reliable of the serological methods used in this study. The study shows that the majority of B. gingivalis strains are fimbriate, and that these fimbriae are immunologically related to the fimbriae of B. gingivalis strain 381. Molecular heterogeneity of fimbrilin was discovered by the immunoblotting analysis, when different strains were compared. With most of the strains, including strain 381, the antifimbrilin serum reacted with a protein of apparent molecular mass 43 kDa, but with 15 strains the immuno-reactive protein had an apparent molecular mass of 46 kDa.