Species identification of Oetzi's clothing with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based on peptide pattern similarities of hair digests
Species identification of Oetzi's clothing with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based on peptide pattern similarities of hair digests
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DOI：
10.1002/rcm.3679
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发表时间：
2008-09-01
影响因子：
2
通讯作者：
Pitra, Christian
中科院分区：
文献类型：
--
作者：
Hollemeyer, Klaus;Altmeyer, Wolfgang;Pitra, Christian
从1991年发现的5300多年前的提洛尔木乃伊（也被称为冰人或奥茨）中鉴定古代生物样本是非常困难的。用一种特殊的基因组学方法，对尚未确诊的四个木乃伊服饰上的动物毛发样品进行了种属鉴定。Ha 43/91/1.30和Ha 6/91.，从他的皮毛上取下的两个样本和从他的腿上取下的一个样本Ha 5/91被分配给绵羊。他的鹿皮鞋Ha 2/91的鞋面皮革是用牛制成的。尽管这些样本的年龄巨大，部分（生物）化学改变，可靠的鉴定是可能的，使用最近开发的基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）为基础的分析方法。该方法完全基于蛋白质的分析，并使用直接来自胰蛋白酶消化的毛发生长素的微量肽，而无需任何分离或富集步骤。未知物种通过将其肽离子模式与存储在现有数据库中的已知光谱进行比较来识别。因此，相关距离，欧几里德距离的一种形式，和推导出的参数被用来测量相似性。如果存在一个以上的潜在匹配，则使用特定的诊断肽离子来逐步排除不正确的匹配。这些离子对于目、科、亚科/属和/或甚至物种是特异性的。肽质量指纹数据与来自碰撞诱导解离光谱（组合的MS & MS/MS）的那些相结合，用于用MASCOT搜索引擎和NCBI数据库进行解释，以找到毛发蛋白的潜在亲本。对于该技术，选定的前体离子被鉴定为特异性诊断肽离子。版权所有（C）2008约翰威利父子有限公司
ldentification of ancient biological samples from the 1991-discovered and more than 5300-year-old Tyrolean mummy, also called iceman or Oetzi, is very difficult. The species of origins of four animal-hair-bearing samples of the accoutrement of the mummy not yet diagnosed were identified by a special pioteomics method. Ha 43/91/1.30 and Ha 6/91., two samples from his coat, and Ha 5/91, a sample from his leggings, were assigned to sheep. The upper leather of his moccasins, Ha 2/91, was made from cattle. Despite the enormous age of these samples with partial (bio)chemical alterations, reliable identification was possible using a recently developed matrix-assisted laser desorption/ionization time-of-flight mass spectrometric (MALDI-TOF MS)-based analytical method. The method is exclusively based on the analysis of proteins and uses minute amounts of peptides directly derived from tryptic hair digests without any separation or enrichment steps. Unknown species are identified by comparison of their peptide ion patterns with known spectra stored in existing databases. Hereby, the correlation distance, a form of Euclidean distance, and deduced parameters are used to measure similarities. If more than one potential hit remains, specific diagnostic peptide ions are used to stepwise exclude incorrect matches. These ions are specific for orders, families, subfamilies/genera and/or even species. Peptide mass fingerprinting data combined with those from collision-induced dissociation spectra (combined MS & MS/MS) were used for interpretation with the MASCOT search engine and the NCBI database to find the potential parentage of hair proteins. For this technique, selected precursor ions were identified as specific diagnostic peptide ions. Copyright (C) 2008 John Wiley & Sons, Ltd.