PRENATAL HALOPERIDOL TREATMENT DECREASES NERVE GROWTH-FACTOR RECEPTOR AND MESSENGER-RNA IN NEONATE RAT FOREBRAIN
PRENATAL HALOPERIDOL TREATMENT DECREASES NERVE GROWTH-FACTOR RECEPTOR AND MESSENGER-RNA IN NEONATE RAT FOREBRAIN
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DOI：
10.1016/0304-3940(91)90620-9
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发表时间：
1991-10-14
影响因子：
2.5
通讯作者：
CASTRO, R
中科院分区：
文献类型：
--
作者：
ALBERCH, J;BRITO, B;CASTRO, R
妊娠大鼠每天接受氟哌啶醇（2 mg/kg）给药，持续11天，直至出生前1天。本实验测定了2日龄大鼠前脑神经生长因子（NGF）及其受体（NGFR）和NGFR-mRNA的含量。使用北方印迹分析，我们观察到NGFR-mRNA的减少。此外，在结合研究中，处理大鼠的K（d）和B（max）低于对照组，但仅在低亲和力结合位点。而氟哌啶醇处理后NGF及其mRNA无明显变化。总之，我们的研究结果表明，产前氟哌啶醇治疗可以修改前脑细胞的发展，通过改变NGFR的表达。
Pregnant rats were treated daily with haloperidol (2 mg/kg) for 11 days until 1 day before birth. The levels of nerve growth factor (NGF), its receptor (NGFR) and NGFR-mRNA were measured in forebrain of 2-day old postnatal rats. Using Northern blot analysis, we observed a decrease in NGFR-mRNA. Furthermore, in binding studies, K(d) and B(max) of treated rats were lower than in controls, but only in the low affinity binding sites. However NGF and its mRNA did not change after haloperidol treatment. In conclusion, our results suggest that prenatal haloperidol treatment can modify the development of forebrain cells, by changing NGFR expression.