Streptomycin mediated biofilm inhibition and suppression of virulence properties in Pseudomonas aeruginosa PAO1
Streptomycin mediated biofilm inhibition and suppression of virulence properties in Pseudomonas aeruginosa PAO1
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DOI：
10.1007/s00253-019-10190-w
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发表时间：
2020-01-01
影响因子：
5
通讯作者：
Kim, Young-Mog
中科院分区：
文献类型：
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作者：
Khan, Fazlurrahman;Lee, Jang-Won;Kim, Young-Mog
铜绿假单胞菌被认为是一种机会致病菌，其抗生素耐药机制之一包括生物膜形成和毒力因子产生。本研究表明，链霉素的亚最小抑菌浓度（sub-MIC）抑制生物膜的形成，并根除已建立的成熟生物膜。亚MIC浓度的链霉素也能够抑制导尿管上生物膜的形成。此外，链霉素的亚MIC减弱了细菌的毒力特性，这一点通过表型和基因表达研究得到证实。链霉素产生抗生物被膜和抗细菌毒力的最佳条件为35 ℃的碱性TSB培养基（pH7.9）。然而，在相似的pH水平和温度下，链霉素在LB培养基中也表现出相当的抗生物膜效力。此外，该条件还改善了链霉素、妥布霉素和四环素对由铜绿假单胞菌的临床分离株形成的生物膜的生物膜抑制和根除特性。本研究的结果为进一步研究链霉素、妥布霉素和四环素在特定培养环境下对生物膜的抑制和分散机制提供了重要依据。
Pseudomonas aeruginosa is known as an opportunistic pathogen whose one of the antibiotic resistance mechanisms includes biofilm formation and virulence factor production. The present study showed that the sub-minimum inhibitory concentration (sub-MIC) of streptomycin inhibited the formation of biofilm and eradicated the established mature biofilm. Streptomycin at sub-MIC was also capable of inhibiting biofilm formation on the urinary catheters. In addition, the sub-MIC of streptomycin attenuated the bacterial virulence properties as confirmed by both phenotypic and gene expression studies. The optimal conditions for streptomycin to perform anti-biofilm and anti-virulence activities were proposed as alkaline TSB media (pH 7.9) at 35 degrees C. However, sub-MIC of streptomycin also exhibited a comparative anti-biofilmefficacy in LB media at similar pH level and temperature. Furthermore, this condition also improved the biofilm inhibition and eradication properties of streptomycin, tobramycin and tetracycline towards the biofilm formed by a clinical isolate of P. aeruginosa. Findings from the present study provide an important insight for further studies on the mechanisms of biofilm inhibition and dispersion of pre-existing biofilm by streptomycin as well as tobramycin and tetracycline under a specific culture environment.