Protection against lethal challenge of BALB/c mice by passive transfer of monoclonal antibodies to five glycoproteins of herpes simplex virus type 2
Protection against lethal challenge of BALB/c mice by passive transfer of monoclonal antibodies to five glycoproteins of herpes simplex virus type 2
复制标题
通过将单克隆抗体被动转移至 2 型单纯疱疹病毒的五种糖蛋白来保护 BALB/c 小鼠免受致命攻击
作者：
N. Balachandran;S. Bacchetti;W. Rawls
单克隆抗体分泌的6个杂交瘤和识别的糖蛋白gC，gAB，gD，gE，和gF的单纯疱疹病毒2型的抗原位点的能力，以保护BALB/c小鼠从致命的病毒感染进行了检查。在用10倍于50%致死剂量的病毒进行足垫攻击前3小时，腹膜内给予单个糖蛋白的单克隆抗体，可保护35%至75%的小鼠，除了两种单克隆抗体中的一种识别gC上的抗原。该抗体在体外不能中和病毒，并能像补体充足的BALB/c小鼠一样有效地保护补体第五组分缺陷的A/J小鼠。通过抗体依赖性细胞介导的细胞溶解评估的单克隆抗体的保护和滴度之间存在良好的相关性。结果表明，任何糖蛋白都可以作为保护性免疫应答的抗原。此外，数据与抗体依赖性细胞介导的细胞溶解机制介导的保护作用一致。
Monoclonal antibodies secreted by six hybridomas and recognizing antigenic sites on glycoproteins gC, gAB, gD, gE, and gF of herpes simplex virus type 2 were examined for their ability to protect BALB/c mice from lethal infection by the virus. Administration of monoclonal antibodies to individual glycoproteins intraperitoneally 3 h before footpad challenge with 10 times the 50% lethal dose of virus protected between 35 and 75% of the mice, except for one of two monoclonal antibodies recognizing antigens on gC. The antibodies did not neutralize virus in vitro and protected A/J mice deficient in the fifth component of complement as efficiently as complement-sufficient BALB/c mice. A good correlation was found between protection and titers of monoclonal antibodies assessed by antibody-dependent cell-mediated cytolysis. The results indicate that any of the glycoproteins can serve as antigens for a protective immune response. In addition, the data are compatible with protection being mediated by an antibody-dependent cell-mediated cytolysis mechanism.
单纯疱疹病毒 1 型糖蛋白参与细胞介导的免疫。
DOI：
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发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Carter,VC;Schaffer,PA;Tevethia,SS
通讯作者：
Tevethia,SS