Study on the bacterial midgut microbiota associated to different Brazilian populations of Lutzomyia longipalpis (Lutz & Neiva) (Diptera: Psychodidae)
Study on the bacterial midgut microbiota associated to different Brazilian populations of Lutzomyia longipalpis (Lutz & Neiva) (Diptera: Psychodidae)
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DOI：
10.1590/s1519-566x2008000500016
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发表时间：
2008-10-01
影响因子：
1.8
通讯作者：
Oliveira, Sandra M.P. de
中科院分区：
文献类型：
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作者：
Gouveia, Cheryl;Asensi, Marise D.;Oliveira, Sandra M.P. de
与三个巴西Lutzomyia longipalpis（Lutz & Neiva）种群中肠相关的细菌群落，其中两个来自内脏利什曼病流行区（巴伊亚州的Jacobina和马兰豪州的圣刘易斯），一个来自非流行区（米纳斯吉拉斯州的Lapinha洞穴）。从每个种群中分离出5个组，每组35只雌性;每个采集区共分析了175只雌性。种属鉴定以分子生物学和传统细菌学方法为基础。所有细菌要么属于非肠杆菌科，如不动杆菌属、伯克霍尔德氏菌属、黄单胞菌属、假单胞菌属和寡养单胞菌属，要么属于肠杆菌科，如柠檬酸杆菌属、肠杆菌属、埃希氏菌属、克雷伯氏菌属、沙雷氏菌属、泛菌属、摩根氏菌属和Weeksella。寡养单胞菌被发现与所有三个人口的研究。此外，沙雷氏菌属，这是有据可查的实验室污染物的昆虫，只在Jacobina人口。我们还讨论了细菌在昆虫肠道定殖对病原体发育和传播的影响。
The bacterial community associated with the midgut of three Brazilian Lutzomyia longipalpis (Lutz & Neiva) populations, two from endemic areas for visceral leishmaniasis (Jacobina, Bahia State and Sao Luis, Maranhao State) and one from a non-endemic area (Lapinha Cave, Minas Gerais State), was identified. Five groups, 35 females each, from each population were separated; a total of 175 females per collecting area were analyzed. The species identification was based on molecular and traditional bacteriological methods. All bacteria were either affiliated to non-Enterobacteriaceae, such as Acinetobacter, Burkholderia, Flavimonas, Pseudomonas and Stenotrophomonas, or and to Enterobacteriaceae, such as Citrobacter, Enterobacter, Escherichia, Klebsiella, Serratia, Pantoea, Morganella and Weeksella. Stenotrophomonas was found to be associated with all three populations studied. In addition, Serratia spp., which are well documented as laboratory contaminant of insects, were detected only in the Jacobina population. We also discuss the impact of the colonization of insect gut by bacteria on the development and transmission of pathogens.