AUGMENTATION OF NK ACTIVITY AFTER ORAL-ADMINISTRATION OF A TRADITIONAL CHINESE MEDICINE, XIAO-CHAI-HU-TANG (SHOSAIKO-TO)
AUGMENTATION OF NK ACTIVITY AFTER ORAL-ADMINISTRATION OF A TRADITIONAL CHINESE MEDICINE, XIAO-CHAI-HU-TANG (SHOSAIKO-TO)
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DOI：
10.3109/08923979409029899
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发表时间：
1994-01-01
影响因子：
3.3
通讯作者：
NOMOTO, K
中科院分区：
文献类型：
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作者：
KANEKO, M;KAWAKITA, T;NOMOTO, K
我们已经证明，传统中药小柴胡汤（日本名：Shosaiko-to）增强小鼠自然杀伤细胞（NK）（1）的活性。外周血和肝脏中NK活性的最大增强在给药后12小时观察到。Shosaiko-to剂量依赖性地增强NK活性。在500 mg/kg剂量下，增强变得显著阳性，在1000 mg/kg剂量下观察到最大效应。NK活性的增强首先出现在肝脏中，从服用Shosaiko-to后6小时开始，随后在外周血中，从服用后12小时开始可检测到。Shosaiko-to对NK细胞的激活可能发生在肝脏中，随后激活的NK细胞可能被供应到外周血。Shosaiko-to处理后细胞表面标志物（去唾液酸GM 1、CD 3、CD 4、CD 8）的百分比变化几乎未检测到，但在细胞毒性试验前，抗去唾液酸GM 1抗体处理消除了Shosaiko-to诱导的NK活性增强。此外，在Shosaiko-to处理的小鼠中未检测到对P-815靶细胞的细胞毒性活性。Shosaiko-to增强NK细胞活性可能是通过激活具有去唾液酸GM 1（+）表型的经典NK细胞而实现的，提示增强肝脏NK细胞活性是Shosaiko-to治疗慢性病毒性肝炎的作用机制之一。
We have shown that a traditional Chinese medicine, Xiao-chai-hu-tang (Japanese name: Shosaiko-to) augments natural killer (NK)(1) activity in mice. The maximum augmentation of NK activity in the peripheral blood and liver was observed at 12 hr after administration of Shosaiko-to. NK activity was augmented by Shosaiko-to dose-dependently. The augmentation became significantly positive at a dose of 500 mg/kg, and the maximum effect was observed at a dose of 1000 mg/kg. The augmentation of NK activity appeared at first in the liver from 6 hr after administration of Shosaiko-to and became detectable later in the peripheral blood from 12 hr after the administration. Activation of NK cells by Shosaiko-to may occur in the liver and subsequently the activated NK cells may be supplied to the peripheral blood. Changes in percentages of cell surface markers (asialo GM1, CD3, CD4, CD8) after Shosaiko-to treatment were hardly detected, but augmentation of NK activity induced by Shosaiko-to was abrogated by anti-asialo GM1 antibody treatment before the cytotoxicity assay. In addition, cytotoxic activity to P-815 target cells was not detected in Shosaiko-to treated mice. Augmentation of NK activity by Shosaiko-to is probably mediated by functional activation of classical NK cells of asialo GM1(+) phenotype.These results suggest that augmentation of NK activity in the liver is one of mechanisms involved in clinical efficacy of Shosaiko-to in patients with virus chronic hepatitis.