DISTRIBUTION OF BIG-TAU IN THE CENTRAL-NERVOUS-SYSTEM OF THE ADULT AND DEVELOPING RAT
DISTRIBUTION OF BIG-TAU IN THE CENTRAL-NERVOUS-SYSTEM OF THE ADULT AND DEVELOPING RAT
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DOI：
10.1002/cne.903580209
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发表时间：
1995-07-24
影响因子：
2.5
通讯作者：
FISCHER, I
中科院分区：
文献类型：
--
作者：
BOYNE, LJ;TESSLER, A;FISCHER, I
神经元形态和功能的多样性与各种微管相关蛋白（MAPs）的特异性表达相关。主要的神经元MAP之一，tau，具有多种异构体形成的选择性剪接和磷酸化的结果，在发育过程中差异表达。大tau蛋白是一种高分子量同种型，含有一个额外的大外显子（4a），主要由周围神经系统（PNS）中的神经元表达。我们在表达载体中克隆了完整的4a外显子，分离了重组蛋白，并产生了对大tau蛋白特异性的抗体，这些抗体用于在发育中的脊髓和成人中枢神经系统（CNS）中定位大tau蛋白。在发育中的脊髓中，大tau蛋白分别在胚胎第18天和出生后第2天首先在背根神经节神经元的中枢投射中和运动神经元中表达。在成年大鼠CNS中，几乎所有延伸到PNS的神经元都表达Big tau，包括所有颅神经运动核和大多数感觉神经节的中枢突起;在这些神经节中，只有嗅觉、前庭和螺旋神经节的双极神经元不表达Big tau。视网膜神经节细胞是唯一的CNS神经元，其过程完全保留在CNS内，表达高水平的Big tau。大tau蛋白的有限和特异性分布与稳定受到巨大剪切力的轴突中微管的作用一致。(C)1995 Wiley-Liss，Inc.
The diversity of neuronal morphology and function is correlated with specific expression of various microtubule associated proteins (MAPs). One of the major neuronal MAPs, tau, has multiple isoforms formed as a result of alternative splicing and phosphorylation that are differentially expressed during development. Big tau is a high molecular weight isoform that contains an additional large exon (4a) and is expressed primarily by neurons in the peripheral nervous system (PNS). We cloned the complete 4a exon in an expression vector, isolated the recombinant protein and produced antibodies specific to Big tau that were used to localize Big tau in the developing spinal cord and in the adult central nervous system (CNS). In developing spinal cord, Big tau is first expressed in the central projections of the dorsal root ganglia neurons and in motor neurons at embryonic day 18 and postnatal day 2, respectively. In the adult rat CNS, almost all neurons that extend processes into the PNS express Big tau, including all cranial nerve motor nuclei and central processes of most sensory ganglia; of these ganglia, only the bipolar neurons of the olfactory, vestibular and spiral ganglia did not express Big tau. Retinal ganglion cells are the only CNS neurons, whose processes remain entirely within the CNS, that express high levels of Big tau. The limited and specific distribution of Big tau is consistent with a role in stabilizing microtubules in axons that are subjected to great shear forces. (C) 1995 Wiley-Liss, Inc.