Porcine kidneys as a source of ECM scaffold for kidney regeneration
Porcine kidneys as a source of ECM scaffold for kidney regeneration
复制标题
猪肾作为肾脏再生 ECM 支架的来源
DOI：
10.1016/j.msec.2015.07.007
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发表时间：
2015-11-01
影响因子：
7.9
通讯作者：
Zhao, Shengtian
中科院分区：
文献类型：
--
作者：
Guan, Yong;Liu, Shuangde;Zhao, Shengtian
目的：目的：制备脱细胞猪肾支架材料，并对其性能进行评价，为肾脏再生研究提供实验平台。然后对肾细胞外基质支架进行血管成像、组织学检查，观察血管通畅性、脱细胞程度。结果：成功制备了猪肾细胞外基质支架。脱细胞肾支架保留完整的微结构，包括肾血管和必要的细胞外基质components.Conclusion：我们已经开发出一种优秀的脱细胞方法，可用于大型器官。这些支架保持其基本成分，并显示完整的血管系统。这代表了使用组织工程技术开发可移植器官的一个步骤。(C)2015 Elsevier B.V.版权所有。
Objective: To produce and examine decellularized kidney scaffolds from porcine as a platform for kidney regeneration research.Methods: Porcine kidneys were decellularized with sodium dodecyl sulfate solution and Triton X-100 after the blood was rinsed. Then the renal ECM scaffolds were examined for vascular imaging, histology to investigate the vascular patency, degree of decellularization.Results: Renal ECM scaffolds of porcine kidneys were successfully produced. Decellularized renal scaffolds retained intact microarchitecture including the renal vasculature and essential extracellular matrix components.Conclusion: We have developed an excellent decellularization method that can be used in large organs. These scaffolds maintain their basic components, and show intact vasculature system. This represents a step toward development of a transplantable organ using tissue engineering techniques. (C) 2015 Elsevier B.V. All rights reserved.