Induced expressions of Rab24 GTPase and LC3 in nerve-injured motor neurons
Induced expressions of Rab24 GTPase and LC3 in nerve-injured motor neurons
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DOI：
10.1016/j.bbrc.2005.09.171
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发表时间：
2005-12-02
影响因子：
3.1
通讯作者：
Kiyama, H
中科院分区：
文献类型：
--
作者：
Egami, Y;Kiryu-Seo, S;Kiyama, H
Rab24是Rab GTPase家族的成员，但其功能尚不清楚。在这里，我们证明了大鼠神经损伤的舌下运动神经元中 Rab24 mRNA 的增加。蛋白酶体抑制剂 (MG132) 处理后，分化的 PC12 细胞中 Rab24 mRNA 的表达也被诱导。 MG132 处理进一步诱导微管相关蛋白轻链 3 (LC3) 的表达，以及 LC3-II 的积累，LC3-II 是 LC3 的加工形式，也是最可靠的自噬标记物。在神经损伤的舌下运动神经元中也观察到 LC3 mRNA 的诱导和 LC3-II 的积累，并且通过免疫组织化学证明了 Rab24 和 LC3 的部分共定位。目前的数据表明，神经损伤促进了自噬样事件，这可能是降解不必要的和错误折叠的蛋白质以回收有限的氨基酸并合成生存和神经再生反应所必需的新蛋白质的重要反应。 (c) 2005 Elsevier Inc. 保留所有权利。
Rab24 is a member of the Rab GTPase family, but its function is unclear. Here, we demonstrated increase in Rab24 mRNA in nerve-injured hypoglossal motor neurons of rats. Expression of Rab24 mRNA was also induced in differentiated PC12 cells following proteasome inhibitor (MG132) treatment. MG132 treatment further induced expression of microtubule-associated protein light chain 3 (LC3), and accumulation of LC3-II, a processed form of LC3 and the most reliable marker for autophagy. Induction of LC3 mRNA and accumulation of LC3-II were also observed in nerve-injured hypoglossal motor neurons, and partial co-localization of Rab24 and LC3 was demonstrated by immunohistochemistry. The present data suggest that nerve injury promotes autophagy-like events, and this may be an important response for degradation of unnecessary and misfolded proteins to recycle limited amino acids, and synthesize new proteins that are necessary for survival and nerve regeneration responses. (c) 2005 Elsevier Inc. All rights reserved.