Cryptochrome-1-dependent execution of programmed cell death induced by singlet oxygen in Arabidopsis thaliana
Cryptochrome-1-dependent execution of programmed cell death induced by singlet oxygen in Arabidopsis thaliana
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DOI：
10.1073/pnas.0608139103
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发表时间：
2006-11-07
影响因子：
11.1
通讯作者：
Apel, Klaus
中科院分区：
文献类型：
--
作者：
Danon, Antoine;Coll, Nuria Sanchez;Apel, Klaus
细胞程序性死亡(PCD)在高等生物的生命周期中起着重要作用。尽管控制PCD的几种调控机制被认为在动植物中是保守的，但依赖光的细胞死亡代表了一种植物特有的PCD形式。PCD的光需求通常与光合作用过程中产生的活性氧有关。支持这一假说的是，过氧化氢和超氧化物被证明参与了PCD反应的触发。在目前的工作中，我们使用拟南芥的条件流感突变体来分析另一种活性氧物种单线态氧对细胞死亡的影响。出乎意料的是，光依赖于单线态氧的释放不足以诱导流感幼苗的PCD，而必须与第二个同时发生的蓝光反应一起作用。这种蓝光特有的PCD触发不能归因于叶绿体内的光合作用反应或氧化还原变化，而是蓝光/UVA特有的光感受器隐色素的激活。单线态氧介导和隐色素依赖的细胞死亡反应与过氧化氢/超氧化物引发的PCD有几个方面的不同。
Programmed cell death (PCD) plays an important role during the life cycle of higher organisms. Although several regulatory mechanisms governing PCD are thought to be conserved in animals and plants, light-dependent cell death represents a form of PCD that is unique to plants. The light requirement of PCD has often been associated with the production of reactive oxygen species during photosynthesis. in support of this hypothesis, hydrogen peroxide and superoxide have been shown to be involved in triggering a PCD response. In the present work, we have used the conditional flu mutant of Arabidopsis to analyze the impact of another reactive oxygen species, singlet oxygen, on cell death. Unexpectedly, the light-dependent release of singlet oxygen alone is not sufficient to induce PCD of flu seedlings but has to act together with a second concurrent blue light reaction. This blue-light-specific trigger of PCD could not be attributed to a photosynthetic reaction or redox change within the chloroplast but to the activation of the blue light/UVA-specific photoreceptor cryptochrome. The singlet oxygen-mediated and cryptochrome-dependent cell death response differs in several ways from PCD triggered by hydrogen peroxide/superoxide.